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Product Info Summary
| SKU: | MP02608 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody
SKU/Catalog Number
MP02608
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody catalog # MP02608. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP02608)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EAG-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
MP02608 is reactive to MAPK8 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
15 kDa
Calculated molecular weight
26053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP02608 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human breast tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of JNK1/2/3 phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAPK8 (Source: Uniprot.org, NCBI)
Gene Name
MAPK8
Full Name
Mitogen-activated protein kinase 8
Weight
26053 MW
Superfamily
protein kinase superfamily
Alternative Names
Red fluorescent protein eqFP611;GFP-like chromoprotein; MAPK8 JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c mitogen-activated protein kinase 8 mitogen-activated protein kinase 8|JUN N-terminal kinase|MAP kinase 8|c-Jun N-terminal kinase 1|stress-activated protein kinase 1|stress-activated protein kinase 1c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK8, check out the MAPK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody (MP02608)
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody
Question
We have observed staining in mouse brain. Any tips? Is anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
From what I have seen in literature brain does express MAPK8. From what I have seen in Uniprot.org, MAPK8 is expressed in forebrain, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2020-03-23
Question
My question regarding product MP02608, anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-14
Question
My colleagues were satisfied with the WB result of your anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody. However we have observed positive staining in forebrain cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
From literature, forebrain does express MAPK8. Generally MAPK8 expresses in cytoplasm. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2019-10-31
Question
I see that the anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-24
Question
Would MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
It shows on the product datasheet, MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
Is a blocking peptide available for product anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody (MP02608)?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We do provide the blocking peptide for product anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody (MP02608). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-04
Question
See attached the WB image, lot number and protocol we used for brain using anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
The anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody (MP02608) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
Question
Does anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 work for IF with brain?
J. Edwards
Verified customer
Asked: 2018-02-02
Answer
According to the expression profile of brain, MAPK8 is highly expressed in brain. So, it is likely that anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 will work for IF with brain.
Boster Scientific Support
Answered: 2018-02-02
Question
Do you have a BSA free version of anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 available?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-05
Question
I am interested in to test anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 on human brain for research purposes, then I may be interested in using anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
The products we sell, including anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody MP02608. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-03
Question
We ordered your anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody for ICC on cervix carcinoma in a previous project. I am using rat, and We are going to use the antibody for IF next. We are interested in examining cervix carcinoma as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Krishna
Verified customer
Asked: 2016-03-02
Answer
I looked at the website and datasheets of our anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody and it seems that MP02608 has been tested on rat in both ICC and IF. Thus MP02608 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-03-02
Question
Is this MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody reactive to the isotypes of MAPK8?
N. Banerjee
Verified customer
Asked: 2015-02-19
Answer
The immunogen of MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody is A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-19
Question
I was wanting to use your anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
R. Roberts
Verified customer
Asked: 2014-04-09
Answer
You can see on the product datasheet, MP02608 anti-Phospho-JNK1/2/3 (T183+T183+T221) Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-09
