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Product Info Summary
| SKU: | P00676 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00676
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal Antibody catalog # P00676. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00676)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IBC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Hsp27 (S78)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00676 is reactive to HSPB1 in Human
Reconstitution
Observed Molecular Weight
185 kDa
Calculated molecular weight
22783 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00676 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: A431 cell, A431 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin.
Protein Target Info & Infographic
Gene/Protein Information For HSPB1 (Source: Uniprot.org, NCBI)
Gene Name
HSPB1
Full Name
Heat shock protein beta-1
Weight
22783 MW
Superfamily
small heat shock protein (HSP20) family
Alternative Names
Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28; HSPB1 CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 heat shock protein family B (small) member 1 heat shock protein beta-1|28 kDa heat shock protein|epididymis secretory protein Li 102|estrogen-regulated 24 kDa protein|heat shock 27 kDa protein|heat shock 27kD protein 1|heat shock 27kDa protein 1|stress-responsive protein 27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB1, check out the HSPB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal Antibody (P00676)
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