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Product Info Summary
| SKU: | P14495 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194
SKU/Catalog Number
P14495
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (Catalog # P14495). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (Boster Biological Technology, Pleasanton CA, USA, Catalog # P14495)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM194
Isotype
Rabbit IgG
Immunogen
A phospho-peptide corresponding to the Phospho-Histone H4 (pSer1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross-reactivity with Histone H2A phosphorylated at Serine 1.
Reactive Species
P14495 is reactive to HIST1H4A in Human, Vertebrates
Reconstitution
Calculated molecular weight
11367 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P14495 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 ug/mL - 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Figure 1. Specificity Test result
RM194 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
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Figure 2. ICC result
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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Figure 3. ICC result
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Protein Target Info & Infographic
Gene/Protein Information For HIST1H4A (Source: Uniprot.org, NCBI)
Gene Name
HIST1H4A
Full Name
Histone H4
Weight
11367 MW
Superfamily
histone H4 family
Alternative Names
Histone H4;HIST1H4A;H4/A, H4FA;HIST1H4B;H4/I, H4FI;HIST1H4C;H4/G, H4FG;HIST1H4D;H4/B, H4FB;HIST1H4E;H4/J, H4FJ;HIST1H4F;H4/C, H4FC;HIST1H4H;H4/H, H4FH;HIST1H4I;H4/M, H4FM;HIST1H4J;H4/E, H4FE;HIST1H4K;H4/D, H4FD;HIST1H4L;H4/K, H4FK;HIST2H4A;H4/N, H4F2, H4FN, HIST2H4;HIST2H4B;H4/O, H4FO;HIST4H4; H4C1 H4-161, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A, H4C1 H4 clustered histone 1 histone H4|H4 histone family, member A|histone 1, H4a|histone cluster 1 H4 family member a|histone cluster 1, H4a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H4A, check out the HIST1H4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 (P14495)
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194
Question
I would like using your anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal antibody, Clone#RM194 for amyloid fiber formation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
S. Krishna
Verified customer
Asked: 2016-11-18
Answer
I appreciate your inquiry. This P14495 anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal antibody, Clone#RM194 is validated on hela cells. It is guaranteed to work for ELISA, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-18
