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Product Info Summary
| SKU: | A00257T433 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-E2F-1 (T433) Antibody
SKU/Catalog Number
A00257T433
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-E2F-1 (T433) Antibody catalog # A00257T433. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-E2F-1 (T433) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00257T433)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. AA range:388-437
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00257T433 is reactive to E2F1 in Human, Mouse
Reconstitution
Calculated molecular weight
46920 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00257T433 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For E2F1 (Source: Uniprot.org, NCBI)
Gene Name
E2F1
Full Name
Transcription factor E2F1
Weight
46920 MW
Superfamily
E2F/DP family
Alternative Names
Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3; E2F1 E2F-1, RBAP1, RBBP3, RBP3 E2F transcription factor 1 transcription factor E2F1|PBR3|PRB-binding protein E2F-1|RBAP-1|RBBP-3|retinoblastoma-associated protein 1|retinoblastoma-binding protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F1, check out the E2F1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-E2F-1 (T433) Antibody (A00257T433)
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