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Product Info Summary
| SKU: | P03300 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-DAPK3 (T265) Antibody
View all DAPK3/ZIPK Antibodies
SKU/Catalog Number
P03300
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-DAPK3 (T265) Antibody catalog # P03300. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-DAPK3 (T265) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03300)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03300 is reactive to DAPK3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
52536 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03300 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1, DAPK3 (Phospho- Thr265) polyclonal antibody (red) was diluted at 1:200 (4° overnight). α-tubulin Monoclonal antibody (8F11) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK3 (Phospho-Thr265) antibody
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Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HUVEC cells, using DAPK3 (Phospho-Thr265) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Protein Target Info & Infographic
Gene/Protein Information For DAPK3 (Source: Uniprot.org, NCBI)
Gene Name
DAPK3
Full Name
Death-associated protein kinase 3
Weight
52536 MW
Superfamily
protein kinase superfamily
Alternative Names
Death-associated protein kinase 3;DAP kinase 3;2.7.11.1 ;DAP-like kinase;Dlk;MYPT1 kinase;Zipper-interacting protein kinase;ZIP-kinase;DAPK3;ZIPK; DAPK3 DLK, ZIP, ZIPK death associated protein kinase 3 death-associated protein kinase 3|DAP kinase 3|DAP-like kinase|MYPT1 kinase|ZIP-kinase|zipper-interacting protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAPK3, check out the DAPK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-DAPK3 (T265) Antibody (P03300)
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No publications found for P03300
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-DAPK3 (T265) Antibody
Question
We are currently using anti-Phospho-DAPK3 (T265) antibody P03300 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-Phospho-DAPK3 (T265) antibody (P03300) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
I am interested in to test anti-Phospho-DAPK3 (T265) antibody P03300 on mouse brain for research purposes, then I may be interested in using anti-Phospho-DAPK3 (T265) antibody P03300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-Phospho-DAPK3 (T265) antibody P03300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
Is this P03300 anti-Phospho-DAPK3 (T265) antibody reactive to the isotypes of DAPK3?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The immunogen of P03300 anti-Phospho-DAPK3 (T265) antibody is Synthesized peptide derived from human DAPK3 around the phosphorylation site of T265. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-04
