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Product Info Summary
| SKU: | A00149T286-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Cyclin D1 (T286) CCND1 Antibody
SKU/Catalog Number
A00149T286-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Cyclin D1 (T286) CCND1 Antibody catalog # A00149T286-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cyclin D1 (T286) CCND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00149T286-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286. AA range:246-295
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00149T286-1 is reactive to CCND1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
33729 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00149T286-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Cyclin D1 (T286) polyclonal antibody diluted at 1:500
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Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) polyclonal antibody diluted at 1:500
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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200
Protein Target Info & Infographic
Gene/Protein Information For CCND1 (Source: Uniprot.org, NCBI)
Gene Name
CCND1
Full Name
G1/S-specific cyclin-D1
Weight
33729 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-D1;B-cell lymphoma 1 protein;BCL-1;BCL-1 oncogene;PRAD1 oncogene;CCND1;BCL1, PRAD1; CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND1, check out the CCND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Cyclin D1 (T286) CCND1 Antibody (A00149T286-1)
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