Product Info Summary
SKU: | P05832 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho Cyclin C (Ser275) CCNC Antibody
SKU/Catalog Number
P05832
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Cyclin C (Ser275) CCNC Antibody (Catalog# P05832). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Cyclin C (Ser275) CCNC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05832)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Cyclin C around the phosphorylation site of Ser275. Immunogen sequence location: 234-283
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P05832 is reactive to CCNC in Human, Mouse, Rat
Applications
P05832 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
33243 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
![CCNC primary antibodies P05832 WB testing CCNC primary antibodies P05832 WB testing](https://www.bosterbio.com/media/catalog/product/C/C/CCNC-primary-antibodies-P05832-WB-testing.jpg)
Click image to see more details
Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using Cyclin C (Phospho-Ser275) Antibody. The lane on the right is blocked with the phospho peptide.
![CCNC primary antibodies P05832 IHC testing CCNC primary antibodies P05832 IHC testing](https://www.bosterbio.com/media/catalog/product/C/C/CCNC-primary-antibodies-P05832-IHC-testing.jpg)
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cyclin C (Phospho-Ser275) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For CCNC (Source: Uniprot.org, NCBI)
Gene Name
CCNC
Full Name
Cyclin-C
Weight
33243 MW
Superfamily
cyclin family
Alternative Names
CycC; cyclin C; cyclin-C; hSRB11; SRB11 homolog CCNC CycC, SRB11, hSRB11 cyclin C cyclin-C|SRB11 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNC, check out the CCNC Infographic
![CCNC infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho Cyclin C (Ser275) CCNC Antibody (P05832)
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No publications found for P05832
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Customer Q&As
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4 Customer Q&As for Anti-Phospho Cyclin C (Ser275) CCNC Antibody
Question
I see that the anti-Phospho Cyclin C (Ser275) antibody P05832 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-Phospho Cyclin C (Ser275) antibody P05832 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-Phospho Cyclin C (Ser275) antibody (P05832) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
I have a question about product P05832, anti-Phospho Cyclin C (Ser275) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P05832 anti-Phospho Cyclin C (Ser275) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-01
Question
Would P05832 anti-Phospho Cyclin C (Ser275) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Gonzalez
Verified customer
Asked: 2016-02-19
Answer
It shows on the product datasheet, P05832 anti-Phospho Cyclin C (Ser275) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-02-19