Product Info Summary
SKU: | A02318-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PGM1 Antibody Picoband™
SKU/Catalog Number
A02318-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PGM1 Antibody Picoband™ catalog # A02318-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PGM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02318-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PGM1 recombinant protein (Position: M1-Q550). Human PGM1 shares 97.3% and 96.5% amino acid (aa) sequence identity with mouse and rat PGM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02318-2 is reactive to PGM1 in Human, Mouse, Rat
Applications
A02318-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
64 kDa
Calculated molecular weight
Background of PGM1
Phosphoglucomutase-1 is an enzyme that in humans is encoded by the PGM1 gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a02318 2 pgm1 primary antibodies wb testing 1 a02318 2 pgm1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02318-2-pgm1-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of PGM1 using anti-PGM1 antibody (A02318-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat skin tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse skin tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM1 antigen affinity purified polyclonal antibody (Catalog # A02318-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGM1 at approximately 64 kDa. The expected band size for PGM1 is at 61 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PGM1 (Source: Uniprot.org, NCBI)
Gene Name
PGM1
Full Name
Phosphoglucomutase-1
Weight
Superfamily
phosphohexose mutase family
Alternative Names
EC 5.4.2; EC 5.4.2.2; Glucose phosphomutase 1; GSD14; PGM 1; phosphoglucomutase 1; phosphoglucomutase-1 PGM1 CDG1T, GSD14 phosphoglucomutase 1 phosphoglucomutase-1|PGM 1|glucose phosphomutase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PGM1, check out the PGM1 Infographic
![PGM1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PGM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGM1 Antibody Picoband™ (A02318-2)
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