Click image to see more details
Product Info Summary
| SKU: | M02686 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PGDH Rabbit Monoclonal Antibody
View all 15-PGDH/HPGD Antibodies
SKU/Catalog Number
M02686
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PGDH Rabbit Monoclonal Antibody catalog # M02686. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02686)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEFF-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prostaglandin dehydrogenase 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02686 is reactive to HPGD in Human
Applications
M02686 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
250 kDa
Calculated molecular weight
12993 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:60
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Prostaglandin dehydrogenase 1 expression in SW480 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HPGD (Source: Uniprot.org, NCBI)
Gene Name
HPGD
Full Name
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
Weight
12993 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
15-hydroxyprostaglandin dehydrogenase [NAD+]; 15PGDH; 15-PGDH; EC 1.1.1; EC 1.1.1.141; HPGD; hydroxyprostaglandin dehydrogenase 15-(NAD); NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; PGDH1; PGDH1PGDH; Prostaglandin dehydrogenase 1; SDR36C1; short chain dehydrogenase/reductase family 36C, member 1 HPGD 15-PGDH, PGDH, PGDH1, PHOAR1, SDR36C1 15-hydroxyprostaglandin dehydrogenase 15-hydroxyprostaglandin dehydrogenase [NAD(+)]|15-hydroxyprostaglandin dehydrogenase (NAD(+))|NAD+-dependent 15-hydroxyprostaglandin dehydrogenase|hydroxyprostaglandin dehydrogenase 15-(NAD)|prostaglandin dehydrogenase 1|short chain dehydrogenase/reductase family 36C member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HPGD, check out the HPGD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HPGD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGDH Rabbit Monoclonal Antibody (M02686)
Hello CJ!
No publications found for M02686
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PGDH Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PGDH Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-PGDH Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-PGDH Monoclonal antibody for IF for human colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon identification?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It shows on the product datasheet, M02686 anti-PGDH Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
Is a blocking peptide available for product anti-PGDH Monoclonal antibody (M02686)?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We do provide the blocking peptide for product anti-PGDH Monoclonal antibody (M02686). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-09
Question
Will anti-PGDH Monoclonal antibody M02686 work on feline WB with placenta?
D. Evans
Verified customer
Asked: 2019-03-07
Answer
Our lab technicians have not tested anti-PGDH Monoclonal antibody M02686 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PGDH Monoclonal antibody M02686 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
We are currently using anti-PGDH Monoclonal antibody M02686 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
The anti-PGDH Monoclonal antibody (M02686) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-05
Question
My question regarding product M02686, anti-PGDH Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02686 anti-PGDH Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-07
Question
Is there a BSA free version of anti-PGDH Monoclonal antibody M02686 available?
P. Jha
Verified customer
Asked: 2014-03-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PGDH Monoclonal antibody M02686 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-19
Question
See below the WB image, lot number and protocol we used for colon using anti-PGDH Monoclonal antibody M02686. Please let me know if you require anything else.
A. Yang
Verified customer
Asked: 2013-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-13
