Product Info Summary
SKU: | A00218-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PGC Antibody Picoband™
View all Pepsinogen C/PGC/Progastricsin Antibodies
SKU/Catalog Number
A00218-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PGC Antibody Picoband™ catalog # A00218-1. Tested in ELISA, IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PGC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00218-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human PGC recombinant protein (Position: Y145-N356). Human PGC shares 69.5% and 70% amino acid (aa) sequence identity with mouse and rat PGC, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00218-1 is reactive to PGC in Human, Mouse, Rat
Applications
A00218-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
52588 MW
Background of Pepsinogen C/PGC/Progastricsin
Progastricsin also known as pepsinogen C or pepsinogen II is a pepsinogen precursor of the enzyme gastricsin that in humans is encoded by the PGC gene. This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human, Mouse, Rat
Immunofluorescence, 5 µg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a00218 1 pgc primary antibodies wb testing 1 a00218 1 pgc primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of PGC using anti-PGC antibody (A00218-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGC antigen affinity purified polyclonal antibody (Catalog # A00218-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGC at approximately 38 kDa. The expected band size for PGC is at 42,32,38 kDa.
![a00218 1 pgc primary antibodies ihc testing 2 a00218 1 pgc primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of PGC using anti-PGC antibody (A00218-1).
PGC was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGC Antibody (A00218-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a00218 1 pgc primary antibodies ihc testing 3 a00218 1 pgc primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of PGC using anti-PGC antibody (A00218-1).
PGC was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGC Antibody (A00218-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a00218 1 pgc primary antibodies ihc testing 4 a00218 1 pgc primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of PGC using anti-PGC antibody (A00218-1).
PGC was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGC Antibody (A00218-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a00218 1 pgc primary antibodies if testing 5 a00218 1 pgc primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of PGC using anti-PGC antibody (A00218-1).
PGC was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGC Antibody (A00218-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a00218 1 pgc primary antibodies if testing 6 a00218 1 pgc primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of PGC using anti-PGC antibody (A00218-1).
PGC was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGC Antibody (A00218-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a00218 1 pgc primary antibodies fcm testing 7 a00218 1 pgc primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of JK cells using anti-PGC antibody (A00218-1).
Overlay histogram showing JK cells stained with A00218-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGC Antibody (A00218-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![a00218 1 pgc primary antibodies fcm testing 8 a00218 1 pgc primary antibodies fcm testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a00218-1-pgc-primary-antibodies-fcm-testing-8.png)
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Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PGC antibody (A00218-1).
Overlay histogram showing HepG2 cells stained with A00218-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PGC Antibody (A00218-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PGC (Source: Uniprot.org, NCBI)
Gene Name
PGC
Full Name
Gastricsin
Weight
52588 MW
Superfamily
peptidase A1 family
Alternative Names
EC 3.4.23; EC 3.4.23.3; FLJ99563; Gastricsin; PEPC; pepsin C; Pepsinogen C; pepsinogen group II; PGC; PGII; preprogastricsin; progastricsin (pepsinogen C) PGC PEPC, PGII progastricsin gastricsin|pepsin C|pepsinogen C|pepsinogen group II|preprogastricsin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PGC, check out the PGC Infographic
![PGC infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PGC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGC Antibody Picoband™ (A00218-1)
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