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Product Info Summary
| SKU: | A11678 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PGA5 Monoclonal Antibody
SKU/Catalog Number
A11678
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PGA5 Monoclonal Antibody catalog # A11678. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGA5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11678)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2C1
Isotype
IgG
Immunogen
Purified recombinant fragment of human PGA5 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11678 is reactive to PGA5 in Human
Reconstitution
Calculated molecular weight
41977 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11678 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Stomach cancer tissues with DAB staining using PGA5 Monoclonal antibody.
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Western Blot (WB) analysis using PGA5 Monoclonal antibody against HepG2 (1) and SMMC-7721 (2) cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PGA5 (Source: Uniprot.org, NCBI)
Gene Name
PGA5
Full Name
Pepsin A-5
Weight
41977 MW
Superfamily
peptidase A1 family
Alternative Names
Pepsin A-4;3.4.23.1;Pepsinogen-4;PGA4; PGA5 Pg5 pepsinogen A5 pepsin A-5|Pepsin A-4|Pepsinogen-4|pepsin A|pepsinogen 5, group I (pepsinogen A)|pepsinogen-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PGA5, check out the PGA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PGA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGA5 Monoclonal Antibody (A11678)
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No publications found for A11678
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PGA5 Monoclonal Antibody
Question
We are currently using anti-PGA5 Monoclonal antibody A11678 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-PGA5 Monoclonal antibody (A11678) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
See attached the WB image, lot number and protocol we used for skeletal muscle using anti-PGA5 Monoclonal antibody A11678. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-PGA5 Monoclonal antibody A11678. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-PGA5 Monoclonal antibody A11678 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-21
Question
I was wanting to use your anti-PGA5 Monoclonal antibody for IHC for human skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skeletal muscle identification?
D. Miller
Verified customer
Asked: 2016-09-30
Answer
It shows on the product datasheet, A11678 anti-PGA5 Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-30
Question
Will A11678 anti-PGA5 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Krishna
Verified customer
Asked: 2014-04-01
Answer
You can see on the product datasheet, A11678 anti-PGA5 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-01
