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Product Info Summary
| SKU: | A06271 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PEX10 Antibody
SKU/Catalog Number
A06271
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PEX10 Antibody (Catalog # A06271). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PEX10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06271)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PEX10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06271 is reactive to PEX10 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
37069 MW
Background of PEX10
Somewhat implicated in the biogenesis of peroxisomes.
Warren D.S., Am. J. Hum. Genet. 63:347-359(1998).
Okumoto K., Hum. Mol. Genet. 7:1399-1405(1998).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06271 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using PEX10 antibody A06271.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PEX10 antiobdy A06271.
Protein Target Info & Infographic
Gene/Protein Information For PEX10 (Source: Uniprot.org, NCBI)
Gene Name
PEX10
Full Name
Peroxisome biogenesis factor 10
Weight
37069 MW
Superfamily
pex2/pex10/pex12 family
Alternative Names
Peroxisome biogenesis factor 10;Peroxin-10;Peroxisomal biogenesis factor 10;Peroxisome assembly protein 10;RING finger protein 69;PEX10;RNF69; PEX10 NALD, PBD6A, PBD6B, RNF69 peroxisomal biogenesis factor 10 peroxisome biogenesis factor 10|RING finger protein 69|peroxin 10|peroxisome assembly protein 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PEX10, check out the PEX10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PEX10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PEX10 Antibody (A06271)
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No publications found for A06271
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PEX10 Antibody
Question
Can you help my question with product A06271, anti-PEX10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06271 anti-PEX10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
Is a blocking peptide available for product anti-PEX10 antibody (A06271)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We do provide the blocking peptide for product anti-PEX10 antibody (A06271). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-06
Question
Will anti-PEX10 antibody A06271 work for WB with c1 segment of cervical spinal cord?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of c1 segment of cervical spinal cord, PEX10 is highly expressed in c1 segment of cervical spinal cord. So, it is likely that anti-PEX10 antibody A06271 will work for WB with c1 segment of cervical spinal cord.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-PEX10 antibody A06271 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The anti-PEX10 antibody (A06271) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-PEX10 antibody A06271. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
I was wanting to use your anti-PEX10 antibody for WB for human c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human c1 segment of cervical spinal cord identification?
D. Jackson
Verified customer
Asked: 2017-11-24
Answer
You can see on the product datasheet, A06271 anti-PEX10 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-24
