Click image to see more details
Product Info Summary
| SKU: | A04614-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PDZ-RhoGEF ARHGEF11 Antibody
SKU/Catalog Number
A04614-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PDZ-RhoGEF ARHGEF11 Antibody catalog # A04614-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDZ-RhoGEF ARHGEF11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04614-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF11. AA range:198-247
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04614-1 is reactive to ARHGEF11 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
167704 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04614-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using PDZ-RhoGEF Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF11 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF11
Full Name
Rho guanine nucleotide exchange factor 11
Weight
167704 MW
Alternative Names
Rho guanine nucleotide exchange factor 11;PDZ-RhoGEF;ARHGEF11;KIAA0380; ARHGEF11 GTRAP48, PDZ-RHOGEF Rho guanine nucleotide exchange factor 11 rho guanine nucleotide exchange factor 11|Rho guanine exchange factor (GEF) 11|Rho guanine nucleotide exchange factor (GEF) 11|RhoA-specific guanine nucleotide exchange factor|RhoGEF glutamate transport modulator|glutamate transporter EAAT4-associated protein 48
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF11, check out the ARHGEF11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDZ-RhoGEF ARHGEF11 Antibody (A04614-1)
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Customer Q&As
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13 Customer Q&As for Anti-PDZ-RhoGEF ARHGEF11 Antibody
Question
Do you have a BSA free version of anti-PDZ-RhoGEF antibody A04614-1 available?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PDZ-RhoGEF antibody A04614-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-24
Question
Would A04614-1 anti-PDZ-RhoGEF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Krishna
Verified customer
Asked: 2020-04-22
Answer
You can see on the product datasheet, A04614-1 anti-PDZ-RhoGEF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-PDZ-RhoGEF antibody A04614-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The anti-PDZ-RhoGEF antibody (A04614-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PDZ-RhoGEF antibody A04614-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-PDZ-RhoGEF antibody A04614-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
Is this A04614-1 anti-PDZ-RhoGEF antibody reactive to the isotypes of ARHGEF11?
M. Roberts
Verified customer
Asked: 2019-10-11
Answer
The immunogen of A04614-1 anti-PDZ-RhoGEF antibody is Synthesized peptide derived from the N-terminal region of human PDZ-RhoGEF. at AA rangle: 170-250. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-11
Question
I was wanting to use your anti-PDZ-RhoGEF antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can see on the product datasheet, A04614-1 anti-PDZ-RhoGEF antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
I see that the anti-PDZ-RhoGEF antibody A04614-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-19
Question
Would anti-PDZ-RhoGEF antibody A04614-1 work on horse WB with skin?
S. Baker
Verified customer
Asked: 2018-01-03
Answer
Our lab technicians have not tested anti-PDZ-RhoGEF antibody A04614-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PDZ-RhoGEF antibody A04614-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-03
Question
Is a blocking peptide available for product anti-PDZ-RhoGEF antibody (A04614-1)?
P. Zhang
Verified customer
Asked: 2017-08-21
Answer
We do provide the blocking peptide for product anti-PDZ-RhoGEF antibody (A04614-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-21
Question
Will anti-PDZ-RhoGEF antibody A04614-1 work for WB with cervix carcinoma?
R. Jha
Verified customer
Asked: 2017-03-08
Answer
According to the expression profile of cervix carcinoma, ARHGEF11 is highly expressed in cervix carcinoma. So, it is likely that anti-PDZ-RhoGEF antibody A04614-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2017-03-08
Question
Can you help my question with product A04614-1, anti-PDZ-RhoGEF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Mangal
Verified customer
Asked: 2017-01-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04614-1 anti-PDZ-RhoGEF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-27
Question
Our lab want to know about to test anti-PDZ-RhoGEF antibody A04614-1 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-PDZ-RhoGEF antibody A04614-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Baker
Verified customer
Asked: 2017-01-13
Answer
The products we sell, including anti-PDZ-RhoGEF antibody A04614-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-13
