Anti-Pdcd4 phospho S457 Monoclonal Antibody

Pdcd4 antibody

Boster Bio Anti-Pdcd4 phospho S457 Monoclonal Antibody (Catalog # MP01105-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Xenopus.

Product Info Summary

SKU: MP01105-1
Size: 50ug
Reactive Species: Human, Mouse, Rat, Xenopus
Host: Mouse
Application: ELISA, WB

Product Name

Anti-Pdcd4 phospho S457 Monoclonal Antibody

View all Pdcd4 Antibodies

SKU/Catalog Number

MP01105-1

Size

50ug

Form

Lyophilized

Description

Boster Bio Anti-Pdcd4 phospho S457 Monoclonal Antibody (Catalog # MP01105-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Xenopus.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Pdcd4 phospho S457 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01105-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 9G6

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

MP01105-1 is reactive to PDCD4 in Human, Mouse, Rat, Xenopus

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

68 kDa

Calculated molecular weight

55775 MW

Background of Pdcd4

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

MP01105-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IP: 1:1,000 - 1:5,000
WB: 1:2,000 - 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For PDCD4 (Source: Uniprot.org, NCBI)

Gene Name

PDCD4

Full Name

Programmed cell death protein 4

Weight

55775 MW

Superfamily

PDCD4 family

Alternative Names

Pdcd4 pS457 biotin conjugation, Pdcd4 pS457, Death up-regulated gene protein, Dug, H731, Ma3, Neoplastic transformation inhibitor, Neoplastic transformation inhibitor protein, Nuclear antigen H731 Pdcd4|D19Ucl, D19Ucla1, MA-, Ma3, T, Tis|programmed cell death 4|programmed cell death protein 4|protein MA-3|topoisomerase-inhibitor suppressed protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDCD4, check out the PDCD4 Infographic

PDCD4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDCD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
MP01105-1

50 μg

$464

Free Secondary Antibody

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MP01105-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$464.00

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