Product Info Summary
SKU: | M00757 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody
View all Park7/DJ-1 Antibodies
SKU/Catalog Number
M00757
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody catalog # M00757. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00757)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GAE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PARK7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00757 is reactive to PARK7 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
20 kDa
Calculated molecular weight
19891 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00757 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:50
Positive Control
WB: human Hela whole cell, human HEL whole cell, human U251 whole cell, human HepG2 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PARK7 using anti-PARK7 antibody (M00757).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARK7 antigen affinity purified monoclonal antibody (Catalog # M00757) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARK7 at approximately 20 kDa. The expected band size for PARK7 is at 20 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using PARK7 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PARK7 (Source: Uniprot.org, NCBI)
Gene Name
PARK7
Full Name
Protein/nucleic acid deglycase DJ-1
Weight
19891 MW
Superfamily
peptidase C56 family
Alternative Names
Protein deglycase DJ-1 ;DJ-1;3.1.2.- ;3.5.1.- ;Oncogene DJ1;Parkinson disease protein 7;PARK7 ; PARK7 DJ-1, DJ1, GATD2, HEL-S-67p Parkinsonism associated deglycase Parkinson disease protein 7|Parkinson disease (autosomal recessive, early onset) 7|epididymis secretory sperm binding protein Li 67p|maillard deglycase|oncogene DJ1|parkinson protein 7|protein DJ-1|protein deglycase DJ-1|protein/nucleic acid deglycase DJ-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARK7, check out the PARK7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody (M00757)
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No publications found for M00757
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Customer Q&As
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7 Customer Q&As for Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody
Question
Does M00757 anti-PARK7/Dj 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
It shows on the product datasheet, M00757 anti-PARK7/Dj 1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-19
Question
I see that the anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-24
Question
Is there a BSA free version of anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 available?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 work on feline IP with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Our lab technicians have not tested anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 on feline. You can run a BLAST between feline and the immunogen sequence of anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline cervix carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-07
Question
Is a blocking peptide available for product anti-PARK7/Dj 1 Rabbit Monoclonal antibody (M00757)?
S. Kulkarni
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-PARK7/Dj 1 Rabbit Monoclonal antibody (M00757). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
Here is the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-PARK7/Dj 1 Rabbit Monoclonal antibody M00757. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-15
Question
I was wanting to use your anti-PARK7/Dj 1 Rabbit Monoclonal antibody for WB for human cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cajal-retzius cell fetal brain cortex identification?
Z. Anderson
Verified customer
Asked: 2013-04-05
Answer
As indicated on the product datasheet, M00757 anti-PARK7/Dj 1 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-05