Product Info Summary
SKU: | A01726 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Parafibromin CDC73 Antibody
View all CDC73/HRPT2 Antibodies
SKU/Catalog Number
A01726
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Parafibromin CDC73 Antibody catalog # A01726. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parafibromin CDC73 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01726)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Human Parafibromin at AA range: 51-100.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01726 is reactive to CDC73 in Human, Mouse, Rat
Applications
A01726 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
60577 MW
Background of CDC73/HRPT2
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001)J Biol Chem; 276(3): 1961-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of 1) mouse heart, 2) mouse spleen cells using primary antibody diluted at 1: 500 (4℃ overnight) . Secondary antibody: Goat Anti-rabbit IgG IRDye 800 (diluted at 1: 5000, 25℃, 1 hour).
Protein Target Info & Infographic
Gene/Protein Information For CDC73 (Source: Uniprot.org, NCBI)
Gene Name
CDC73
Full Name
Parafibromin
Weight
60577 MW
Superfamily
CDC73 family
Alternative Names
C1orf28hyperparathyroidism 2 (with jaw tumor); CDC73; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae); Cell division cycle protein 73 homolog; chromosome 1 open reading frame 28; HPTJT; HPT-JT; HRPT2; HRPT2FLJ23316; Hyperparathyroidism 2 protein; HYX; Parafibromin CDC73 C1orf28, FIHP, HPTJT, HRPT1, HRPT2, HYX cell division cycle 73 parafibromin|Familial isolated hyperparathyroidism|Paf1/RNA polymerase II complex component|cell division cycle 73 Paf1/RNA polymerase II complex component-like protein|cell division cycle 73, Paf1/RNA polymerase II complex component, homolog|cell division cycle protein 73 homolog|hyperparathyroidism 2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC73, check out the CDC73 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC73: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Parafibromin CDC73 Antibody (A01726)
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No publications found for A01726
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Parafibromin CDC73 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Parafibromin antibody A01726. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
Would anti-Parafibromin antibody A01726 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
According to the expression profile of erythroleukemia, CDC73 is highly expressed in erythroleukemia. So, it is likely that anti-Parafibromin antibody A01726 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2019-07-23
Question
Is a blocking peptide available for product anti-Parafibromin antibody (A01726)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-Parafibromin antibody (A01726). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-Parafibromin antibody A01726 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-17
Question
My question regarding product A01726, anti-Parafibromin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Roberts
Verified customer
Asked: 2018-08-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01726 anti-Parafibromin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-16
Question
Does A01726 anti-Parafibromin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Wu
Verified customer
Asked: 2015-06-10
Answer
As indicated on the product datasheet, A01726 anti-Parafibromin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-10