Product Info Summary
SKU: | A11546-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PANX3 Antibody Picoband®
View all Pannexin-3 Antibodies
SKU/Catalog Number
A11546-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PANX3 Antibody Picoband® catalog # A11546-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PANX3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11546-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PANX3 recombinant protein (Position: M1-P392). Human PANX3 shares 89% and 91.6% amino acid (aa) sequence identity with mouse and rat PANX3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11546-1 is reactive to PANX3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45 kDa
Background of Pannexin-3
The protein encoded by this gene belongs to the innexin family. Innexin family members are known to be the structural components of gap junctions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11546-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human MCF-7 whole cell, human A431 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PANX3 using anti-PANX3 antibody (A11546-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PANX3 antigen affinity purified polyclonal antibody (Catalog # A11546-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PANX3 at approximately 45 kDa. The expected band size for PANX3 is at 45 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PANX3 antibody (A11546-1).
Overlay histogram showing MCF-7 cells stained with A11546-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PANX3 Antibody (A11546-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PANX3 (Source: Uniprot.org, NCBI)
Gene Name
PANX3
Full Name
Pannexin-3
Weight
45 kDa
Superfamily
pannexin family
Alternative Names
gap junction protein pannexin 3; pannexin 3; Pannexin3; Pannexin-3; PANX3; PX3 PANX3 PX3 pannexin 3 pannexin-3|gap junction protein pannexin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PANX3, check out the PANX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PANX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PANX3 Antibody Picoband® (A11546-1)
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