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Product Info Summary
| SKU: | A31791 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PAK5 Antibody
SKU/Catalog Number
A31791
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PAK5 Antibody (Catalog # A31791). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
PAK5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PAK5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31791)
Host
Rabbit
Contents
PAK5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PAK5 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK5. The immunogen is located within amino acids 240 - 290 of PAK5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
It is predicted to not cross-react with other members of the cavin family.
Reactive Species
A31791 is reactive to PAK5 in Human
Applications
A31791 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
80745 MW
Background of PAK7
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PAK5 antibody can be used for the detection of PAK5 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PAK5 in T24 lysate with PAK5 antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of PAK5 in K562 cells with PAK5 antibody at 2 μg/mL.
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Immunofluorescence of PAK5 in K562 cells with PAK5 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PAK5 (Source: Uniprot.org, NCBI)
Gene Name
PAK5
Full Name
Serine/threonine-protein kinase PAK 5
Weight
80745 MW
Superfamily
protein kinase superfamily
Alternative Names
PAK5; PAK7 PAK5 PAK7 p21 (RAC1) activated kinase 5 serine/threonine-protein kinase PAK 5|PAK-5|PAK-7|p21 (RAC1) activated kinase 7|p21 protein (Cdc42/Rac)-activated kinase 7|p21(CDKN1A)-activated kinase 7|p21-activated kinase 5|p21-activated kinase 7|p21CDKN1A-activated kinase 7|protein kinase PAK5|serine/threonine-protein kinase PAK 7|serine/threonine-protein kinase PAK7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK5, check out the PAK5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAK5 Antibody (A31791)
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