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Product Info Summary
| SKU: | A04375 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Pig |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PA28 gamma PSME3 Monoclonal Antibody
View all PA28 Activator gamma Subunit/PSME3 Antibodies
SKU/Catalog Number
A04375
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PA28 gamma PSME3 Monoclonal Antibody catalog # A04375. Tested in WB applications. This antibody reacts with Human, Mouse, Pig.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PA28 gamma PSME3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04375)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG
Immunogen
Purified recombinant human PA28γ protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04375 is reactive to PSME3 in Human, Mouse, Pig
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
29506 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04375 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using PA28gamma Monoclonal antibody against HepG2, Jurkat, K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PSME3 (Source: Uniprot.org, NCBI)
Gene Name
PSME3
Full Name
Proteasome activator complex subunit 3
Weight
29506 MW
Superfamily
PA28 family
Alternative Names
Proteasome activator complex subunit 3;11S regulator complex subunit gamma;REG-gamma;Activator of multicatalytic protease subunit 3;Ki nuclear autoantigen;Proteasome activator 28 subunit gamma;PA28g;PA28gamma;PSME3; PSME3 HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA proteasome activator subunit 3 proteasome activator complex subunit 3|11S regulator complex gamma subunit|11S regulator complex subunit gamma|Ki |Ki nuclear auto|PA28 gamma variant 5|REG gamma-3|activator of multicatalytic protease subunit 3|epididymis secretory protein Li 283|proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)|proteasome activator 28 subunit gamma|proteasome activator 28-gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSME3, check out the PSME3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSME3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PA28 gamma PSME3 Monoclonal Antibody (A04375)
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No publications found for A04375
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-PA28 gamma PSME3 Monoclonal Antibody
Question
Would anti-PA28 gamma Monoclonal antibody A04375 work on pig WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Our lab technicians have not validated anti-PA28 gamma Monoclonal antibody A04375 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PA28 gamma Monoclonal antibody A04375 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-29
Question
Is a blocking peptide available for product anti-PA28 gamma Monoclonal antibody (A04375)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-PA28 gamma Monoclonal antibody (A04375). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
Can you help my question with product A04375, anti-PA28 gamma Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04375 anti-PA28 gamma Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-23
Question
I see that the anti-PA28 gamma Monoclonal antibody A04375 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use to test anti-PA28 gamma Monoclonal antibody A04375 on pig embryonic kidney for research purposes, then I may be interested in using anti-PA28 gamma Monoclonal antibody A04375 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The products we sell, including anti-PA28 gamma Monoclonal antibody A04375, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-02
Question
I was wanting to use your anti-PA28 gamma Monoclonal antibody for WB for pig embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for pig embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
As indicated on the product datasheet, A04375 anti-PA28 gamma Monoclonal antibody has been tested for WB on human, mouse, pig tissues. We have an innovator award program that if you test this antibody and show it works in pig embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-PA28 gamma Monoclonal antibody A04375. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
Would A04375 anti-PA28 gamma Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
It shows on the product datasheet, A04375 anti-PA28 gamma Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-25
Question
Is there a BSA free version of anti-PA28 gamma Monoclonal antibody A04375 available?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PA28 gamma Monoclonal antibody A04375 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-10
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-PA28 gamma Monoclonal antibody A04375. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-29
Question
We are currently using anti-PA28 gamma Monoclonal antibody A04375 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, pig. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
The anti-PA28 gamma Monoclonal antibody (A04375) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-25
Question
Will anti-PA28 gamma Monoclonal antibody A04375 work for WB with embryonic kidney?
K. Li
Verified customer
Asked: 2015-02-26
Answer
According to the expression profile of embryonic kidney, PSME3 is highly expressed in embryonic kidney. So, it is likely that anti-PA28 gamma Monoclonal antibody A04375 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2015-02-26
