Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody

Product Name

Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody

View all p27/Kip1 Antibodies

SKU/Catalog Number

M00173

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody (Catalog # M00173). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) CDKN1B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00173)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: DCS-72.F6 + KIP1/769

Isotype

IgG1, kappa

Immunogen

Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00173 is reactive to CDKN1B in Human, Monkey, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

22073 MW

Background of p27/Kip1

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00173 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)

Alternative Names

Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27;p27Kip1;CDKN1B;KIP1; CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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