Anti-OTUD5 Antibody Picoband™

Product Name

Anti-OTUD5 Antibody Picoband™

View all Otud5 Antibodies

SKU/Catalog Number

A09744-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-OTUD5 Antibody Picoband™ catalog # A09352-1. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OTUD5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09744-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human OTUD5 recombinant protein (Position: R168-D555). Human OTUD5 shares 98.2% and 98.5% amino acid (aa) sequence identity with mouse and rat OTUD5, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09744-1 is reactive to Otud5 in Human, Rat

Applications

A09744-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

52588 MW

Background of Otud5

OTU deubiquitinase 5 is a protein that in humans is encoded by the OTUD5 gene. This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OTUD5 using anti-OTUD5 antibody (A09744-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD5 antigen affinity purified polyclonal antibody (Catalog # A09744-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD5 at approximately 75 kDa. The expected band size for OTUD5 is at 61 kDa.

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Figure 2. IF analysis of OTUD5 using anti-OTUD5 antibody (A09744-1) and anti-Beta Tubulin antibody (M01857-3).
OTUD5 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OTUD5 Antibody (A09744-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Otud5 (Source: Uniprot.org, NCBI)

Alternative Names

OTU domain-containing protein 5 Otud5|AA407879, AI553596, BB114028, DUBA, DXImx46, DXImx46e, Dub, Sfc, Sfc7|OTU domain containing 5|OTU domain-containing protein 5|deubiquinating enzyme A|deubiquitinating enzyme A

For more info on Otud5, check out the Otud5 Infographic

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In this infographic, you will see the following information for Otud5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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