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Product Info Summary
| SKU: | PA1431 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Osteopontin/SPP1 Antibody Picoband®
View all Osteopontin/OPN Antibodies
SKU/Catalog Number
PA1431
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Osteopontin/SPP1 Antibody catalog # PA1431. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Osteopontin/SPP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1431)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin, different from the related mouse sequence by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1431 is reactive to SPP1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
35423 MW
Background of Osteopontin/OPN
Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1431 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of OPN/SPP1 using anti-OPN/SPP1 antibody (PA1431).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN/SPP1 antigen affinity purified polyclonal antibody (Catalog # PA1431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPN/SPP1 at approximately 65 kDa. The expected band size for OPN/SPP1 is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SPP1 (Source: Uniprot.org, NCBI)
Gene Name
SPP1
Full Name
Osteopontin
Weight
35423 MW
Superfamily
osteopontin family
Alternative Names
Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin;SPP1;BNSP, OPN;PSEC0156; SPP1 BNSP, BSPI, ETA-1, OPN secreted phosphoprotein 1 osteopontin|SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|secreted phosphoprotein 1 variant 6|urinary stone protein|uropontin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPP1, check out the SPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Osteopontin/SPP1 Antibody Picoband® (PA1431)
Hello CJ!
PA1431 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo
Aksoy A, Artas G, Sevindik OG. Pak J Med Sci. 2017 May-Jun;33(3):560-565. doi: 10.12669/pjms.333.12559. Predictive value of stathmin-1 and osteopontin expression for taxan resistance in metastatic castrate-resistant prostate cancer
Shan A, Zhou C, He Y, Feng W, Chen G, Zhong G. Ren Fail. 2013;35(1):37-42. Doi: 10.3109/0886022X.2012.741645. Epub 2012 Nov 14. Expression Of Both Matrix Metalloproteinase-2 And Its Tissue Inhibitor-2 In Tunica Media Of Radial Artery In Uremic Pat...
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Customer Q&As
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17 Customer Q&As for Anti-Osteopontin/SPP1 Antibody Picoband®
Question
Would anti-Osteopontin/SPP1 antibody PA1431 work for WB with brain kidney?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
According to the expression profile of brain kidney, SPP1 is highly expressed in brain kidney. So, it is likely that anti-Osteopontin/SPP1 antibody PA1431 will work for WB with brain kidney.
Boster Scientific Support
Answered: 2020-01-30
Question
Our lab used your anti-Osteopontin/SPP1 antibody for IHC on liver in a previous project. I am using human, and We are going to use the antibody for WB next. We want examining liver as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
I have checked the website and datasheets of our anti-Osteopontin/SPP1 antibody and it appears that PA1431 has been validated on human in both IHC and WB. Thus PA1431 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-23
Question
I see that the anti-Osteopontin/SPP1 antibody PA1431 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-26
Question
I was wanting to use your anti-Osteopontin/SPP1 antibody for WB for human brain kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain kidney identification?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, PA1431 anti-Osteopontin/SPP1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
We are currently using anti-Osteopontin/SPP1 antibody PA1431 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The anti-Osteopontin/SPP1 antibody (PA1431) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-09
Question
We were satisfied with the WB result of your anti-Osteopontin/SPP1 antibody. However we have been able to see positive staining in kidney subthalamic nucleus secreted. using this antibody. Is that expected? Could you tell me where is SPP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
From what I have seen in literature, kidney subthalamic nucleus does express SPP1. Generally SPP1 expresses in secreted. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2019-11-12
Question
I have a question about product PA1431, anti-Osteopontin/SPP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1431 anti-Osteopontin/SPP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
Do you have a BSA free version of anti-Osteopontin/SPP1 antibody PA1431 available?
W. Mangal
Verified customer
Asked: 2018-11-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Osteopontin/SPP1 antibody PA1431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-26
Question
I have attached the WB image, lot number and protocol we used for brain kidney using anti-Osteopontin/SPP1 antibody PA1431. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-06
Question
We want using your anti-Osteopontin/SPP1 antibody for dna-templated studies. Has this antibody been tested with western blotting on colo320 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Thanks for your inquiry. This PA1431 anti-Osteopontin/SPP1 antibody is validated on mm231 cell lysate, hela cell lysate, jurkat cell lysate, colo320 cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-20
Question
We need to test anti-Osteopontin/SPP1 antibody PA1431 on human brain kidney for research purposes, then I may be interested in using anti-Osteopontin/SPP1 antibody PA1431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Taylor
Verified customer
Asked: 2018-03-14
Answer
The products we sell, including anti-Osteopontin/SPP1 antibody PA1431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-14
Question
Would anti-Osteopontin/SPP1 antibody PA1431 work on bovine WB with kidney subthalamic nucleus?
J. Parker
Verified customer
Asked: 2017-11-03
Answer
Our lab technicians have not tested anti-Osteopontin/SPP1 antibody PA1431 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Osteopontin/SPP1 antibody PA1431 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine kidney subthalamic nucleus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-03
Question
Does PA1431 anti-Osteopontin/SPP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Wu
Verified customer
Asked: 2017-09-18
Answer
As indicated on the product datasheet, PA1431 anti-Osteopontin/SPP1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-18
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain kidney using anti-Osteopontin/SPP1 antibody PA1431. Let me know if you need anything else.
C. Jones
Verified customer
Asked: 2016-11-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-28
Question
Is a blocking peptide available for product anti-Osteopontin/SPP1 antibody (PA1431)?
M. Zhao
Verified customer
Asked: 2014-10-29
Answer
We do provide the blocking peptide for product anti-Osteopontin/SPP1 antibody (PA1431). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-10-29
Question
We have observed staining in human pituitary. Do you have any suggestions? Is anti-Osteopontin/SPP1 antibody supposed to stain pituitary positively?
Z. Anderson
Verified customer
Asked: 2014-02-10
Answer
From what I have seen in literature pituitary does express SPP1. From what I have seen in Uniprot.org, SPP1 is expressed in amniotic fluid, liver, brain, kidney subthalamic nucleus, placenta, brain kidney, kidney, milk, pituitary, among other tissues. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2014-02-10
Question
Is this PA1431 anti-Osteopontin/SPP1 antibody reactive to the isotypes of SPP1?
P. Anderson
Verified customer
Asked: 2014-01-15
Answer
The immunogen of PA1431 anti-Osteopontin/SPP1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin(28-47aa EEKQLYNKYPDAVATWLNPD), different from the related mouse sequence by six amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-15
