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Product Info Summary
| SKU: | PB9919 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Osteocalcin/BGLAP Antibody
View all Osteocalcin Antibodies
SKU/Catalog Number
PB9919
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Osteocalcin/BGLAP Antibody catalog # PB9919. Tested in IHC applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Osteocalcin/BGLAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9919)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95). Mouse Osteocalcin shares 69% and 68.9% amino acid (aa) sequence identity with human and rat Osteocalcin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9919 is reactive to Bglap in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa, 60 kDa
Calculated molecular weight
10549 MW
Background of Osteocalcin
Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9919 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Positive Control
IHC: mouse tibia tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9919).
Osteocalcin was detected in paraffin-embedded section of mouse tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Osteocalcin Antibody (PB9919) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Bglap (Source: Uniprot.org, NCBI)
Gene Name
Bglap
Full Name
Osteocalcin
Weight
10549 MW
Superfamily
osteocalcin/matrix Gla protein family
Alternative Names
Osteocalcin;Bone Gla protein;BGP;Gamma-carboxyglutamic acid-containing protein;Bglap; BGLAP BGP, OC, OCN bone gamma-carboxyglutamate protein osteocalcin|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bglap, check out the Bglap Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bglap: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Osteocalcin/BGLAP Antibody (PB9919)
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Customer Q&As
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13 Customer Q&As for Anti-Osteocalcin/BGLAP Antibody
Question
Is a blocking peptide available for product anti-Osteocalcin/BGLAP antibody (PB9919)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-Osteocalcin/BGLAP antibody (PB9919). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
Does anti-Osteocalcin/BGLAP antibody PB9919 work for IHC-P with myometrium?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to the expression profile of myometrium, BGLAP is highly expressed in myometrium. So, it is likely that anti-Osteocalcin/BGLAP antibody PB9919 will work for IHC-P with myometrium.
Boster Scientific Support
Answered: 2020-01-17
Question
Will anti-Osteocalcin/BGLAP antibody PB9919 work on horse IHC-P with myometrium?
P. Taylor
Verified customer
Asked: 2019-12-24
Answer
Our lab technicians have not validated anti-Osteocalcin/BGLAP antibody PB9919 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Osteocalcin/BGLAP antibody PB9919 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse myometrium in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-24
Question
Is this PB9919 anti-Osteocalcin/BGLAP antibody reactive to the isotypes of BGLAP?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The immunogen of PB9919 anti-Osteocalcin/BGLAP antibody is E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95). Mouse Osteocalcin shares 69% and 68.9% amino acid (aa) sequence identity with human and rat Osteocalcin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-02
Question
I see that the anti-Osteocalcin/BGLAP antibody PB9919 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-09
Question
Here is the WB image, lot number and protocol we used for myometrium using anti-Osteocalcin/BGLAP antibody PB9919. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
I would like to test anti-Osteocalcin/BGLAP antibody PB9919 on mouse myometrium for research purposes, then I may be interested in using anti-Osteocalcin/BGLAP antibody PB9919 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-Osteocalcin/BGLAP antibody PB9919, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-Osteocalcin/BGLAP antibody PB9919 for mouse tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
The anti-Osteocalcin/BGLAP antibody (PB9919) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-27
Question
I have a question about product PB9919, anti-Osteocalcin/BGLAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9919 anti-Osteocalcin/BGLAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-06
Question
I was wanting to use your anti-Osteocalcin/BGLAP antibody for IHC-P for mouse myometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse myometrium identification?
P. Evans
Verified customer
Asked: 2017-07-19
Answer
As indicated on the product datasheet, PB9919 anti-Osteocalcin/BGLAP antibody has been tested for IHC-P on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse myometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myometrium using anti-Osteocalcin/BGLAP antibody PB9919. Let me know if you need anything else.
Z. Evans
Verified customer
Asked: 2017-07-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-06
Question
Is there a BSA free version of anti-Osteocalcin/BGLAP antibody PB9919 available?
R. Thomas
Verified customer
Asked: 2017-03-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Osteocalcin/BGLAP antibody PB9919 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-03-10
Question
Will PB9919 anti-Osteocalcin/BGLAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Zhao
Verified customer
Asked: 2016-10-10
Answer
As indicated on the product datasheet, PB9919 anti-Osteocalcin/BGLAP antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-10-10
