Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband™

Product Name

Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband™

View all alpha 1-Acid Glycoprotein Antibodies

SKU/Catalog Number

PB9956

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband™ catalog # PB9956. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9956)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ORM1 recombinant protein (Position: Q19-S201). Human ORM1 shares 47.5% and 48.1% amino acid (aa) sequence identity with mouse and rat ORM1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9956 is reactive to ORM1 in Human, Mouse, Rat

Applications

PB9956 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

40-47 kDa

Calculated molecular weight

23512 MW

Background of alpha 1-Acid Glycoprotein

Alpha-1-acid glycoprotein 1 is a protein that in humans is encoded by the ORM1 gene. The structural gene for orosomucoid (ORM1) is assigned to the end of the long arm of chromosome 9 by demonstration of linkage to ABO and AK1. This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.

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Figure 2. IHC analysis of ORM1 using anti-ORM1 antibody (PB9956).
ORM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ORM1 Antibody (PB9956) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For ORM1 (Source: Uniprot.org, NCBI)

Alternative Names

AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; alpha 1Acid Glycoprotein; alpha 1-Acid Glycoprotein; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1 ORM1 AGP-A, AGP1, HEL-S-153w, ORM orosomucoid 1 alpha-1-acid glycoprotein 1|AGP 1|OMD 1|epididymis secretory sperm binding protein Li 153w

For more info on ORM1, check out the ORM1 Infographic

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In this infographic, you will see the following information for ORM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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