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Product Info Summary
| SKU: | A02735 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ORC1 Antibody
SKU/Catalog Number
A02735
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ORC1 Antibody catalog # A02735. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ORC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02735)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ORC1L. AA range:331-380
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02735 is reactive to ORC1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
97350 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02735 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 3T3 cells using ORC1 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using ORC1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ORC1 (Source: Uniprot.org, NCBI)
Gene Name
ORC1
Full Name
Origin recognition complex subunit 1
Weight
97350 MW
Superfamily
ORC1 family
Alternative Names
Origin recognition complex subunit 1;Replication control protein 1;ORC1;ORC1L, PARC1; ORC1 HSORC1L, PARC1, ORC1 origin recognition complex subunit 1 origin recognition complex subunit 1|origin recognition complex, subunit 1 homolog|replication control protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ORC1, check out the ORC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ORC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ORC1 Antibody (A02735)
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No publications found for A02735
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-ORC1 Antibody
Question
Does A02735 anti-ORC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Jones
Verified customer
Asked: 2019-07-30
Answer
You can see on the product datasheet, A02735 anti-ORC1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-30
Question
I see that the anti-ORC1 antibody A02735 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-ORC1 antibody A02735 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-ORC1 antibody (A02735) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ORC1 antibody A02735. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-21
Question
Is a blocking peptide available for product anti-ORC1 antibody (A02735)?
S. Krishna
Verified customer
Asked: 2014-12-23
Answer
We do provide the blocking peptide for product anti-ORC1 antibody (A02735). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-23
