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Product Info Summary
| SKU: | A00737-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-OPRM1 Antibody Picoband®
View all mu Opioid R/OPRM1 Antibodies
SKU/Catalog Number
A00737-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OPRM1 Antibody Picoband® catalog # A00737-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OPRM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00737-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OPRM1 recombinant protein (Position: N33-H321).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00737-2 is reactive to OPRM1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
44.779kDa
Background of mu Opioid R/OPRM1
This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A>G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00737-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human K562 whole cell, human Jurkat whole cell, human U251 whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OPRM1 using anti-OPRM1 antibody (A00737-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPRM1 antigen affinity purified polyclonal antibody (Catalog # A00737-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPRM1 at approximately 70 kDa. The expected band size for OPRM1 is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For OPRM1 (Source: Uniprot.org, NCBI)
Gene Name
OPRM1
Full Name
Mu-type opioid receptor
Weight
44.779kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; MMP1; CLG OPRM1 LMOR, M-OR-1, MOP, MOR, MOR1, OPRM opioid receptor mu 1 mu-type opioid receptor|mu opiate receptor|mu opioid receptor hMOR-1a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OPRM1, check out the OPRM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OPRM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OPRM1 Antibody Picoband® (A00737-2)
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