Anti-OPA1 Antibody Picoband®

OPA1 antibody

Boster Bio Anti-OPA1 Antibody Picoband® catalog # PB9731. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9731
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-OPA1 Antibody Picoband®

View all OPA1 Antibodies

SKU/Catalog Number

PB9731

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OPA1 Antibody Picoband® catalog # PB9731. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OPA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9731)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9731 is reactive to OPA1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

80-100 kDa

Calculated molecular weight

111631 MW

Background of OPA1

Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9731 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human MCF-7 whole cell, human PANC-1 whole cell, human PC-3 whole cell, human T47D whole cell, rat brain tissue, mouse brain tissue, mouse heart tissue, mouse NIH/3T3 whole cell
IHC: Mouse Liver tissue, Rat Kidney tissue, Human Lung Cancer tissue
ICC/IF: U20S cell
FCM: U20S cell

Validation Images & Assay Conditions

Gene/Protein Information For OPA1 (Source: Uniprot.org, NCBI)

Gene Name

OPA1

Full Name

Dynamin-like 120 kDa protein, mitochondrial

Weight

111631 MW

Superfamily

TRAFAC class dynamin-like GTPase superfamily

Alternative Names

Dynamin-like 120 kDa protein, mitochondrial;3.6.5.5;Optic atrophy protein 1;Dynamin-like 120 kDa protein, form S1;OPA1;KIAA0567; OPA1 BERHS, MGM1, MTDPS14, NPG, NTG, largeG OPA1 mitochondrial dynamin like GTPase dynamin-like 120 kDa protein, mitochondrial|dynamin-like guanosine triphosphatase|mitochondrial dynamin-like GTPase|optic atrophy 1 (autosomal dominant)|optic atrophy protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on OPA1, check out the OPA1 Infographic

OPA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for OPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-OPA1 Antibody Picoband®

Question

Our lab used your anti-OPA1 antibody for IF on liver in the past. I am using human, and We intend to use the antibody for IHC-P next. I was wanting to use examining liver as well as corpus callosum in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

I have checked the website and datasheets of our anti-OPA1 antibody and I see that PB9731 has been tested on human in both IF and IHC-P. Thus PB9731 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-27

Question

Is this PB9731 anti-OPA1 antibody reactive to the isotypes of OPA1?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

The immunogen of PB9731 anti-OPA1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-14

Question

We want using your anti-OPA1 antibody for negative regulation of release of cytochrome c from mitochondria studies. Has this antibody been tested with western blotting on skov whole cell lysate? We would like to see some validation images before ordering.

S. Carter

Verified customer

Asked: 2019-12-09

Answer

We appreciate your inquiry. This PB9731 anti-OPA1 antibody is validated on a549 whole cell lysate, skov whole cell lysate, sw620 whole cell lysate, mouse liver tissue, rat kidney tissue, lung cancer tissue, u20s cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-09

Question

Is a blocking peptide available for product anti-OPA1 antibody (PB9731)?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

We do provide the blocking peptide for product anti-OPA1 antibody (PB9731). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-17

Question

Would anti-OPA1 antibody PB9731 work on feline Flow Cytometry with liver?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

Our lab technicians have not tested anti-OPA1 antibody PB9731 on feline. You can run a BLAST between feline and the immunogen sequence of anti-OPA1 antibody PB9731 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-01

Question

My lab would like to test anti-OPA1 antibody PB9731 on mouse brain for research purposes, then I may be interested in using anti-OPA1 antibody PB9731 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

The products we sell, including anti-OPA1 antibody PB9731, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-OPA1 antibody PB9731. Let me know if you need anything else.

P. Evans

Verified customer

Asked: 2019-06-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-20

Question

We have been able to see staining in human liver. What should we do? Is anti-OPA1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

From literature liver does express OPA1. From Uniprot.org, OPA1 is expressed in corpus callosum, brain, liver, among other tissues. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-05-27

Question

Can you help my question with product PB9731, anti-OPA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Brown

Verified customer

Asked: 2019-01-25

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9731 anti-OPA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-25

Question

We are currently using anti-OPA1 antibody PB9731 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

J. Gonzalez

Verified customer

Asked: 2018-10-19

Answer

The anti-OPA1 antibody (PB9731) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-19

Question

Will anti-OPA1 antibody PB9731 work for ICC with brain?

Verified Customer

Verified customer

Asked: 2018-08-01

Answer

According to the expression profile of brain, OPA1 is highly expressed in brain. So, it is likely that anti-OPA1 antibody PB9731 will work for ICC with brain.

Boster Scientific Support

Answered: 2018-08-01

Question

Here is the WB image, lot number and protocol we used for brain using anti-OPA1 antibody PB9731. Please let me know if you require anything else.

O. Lewis

Verified customer

Asked: 2017-09-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-20

Question

I see that the anti-OPA1 antibody PB9731 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-07-11

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-11

Question

Is there a BSA free version of anti-OPA1 antibody PB9731 available?

G. Krishna

Verified customer

Asked: 2017-07-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-OPA1 antibody PB9731 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-04

Question

Our team were happy with the WB result of your anti-OPA1 antibody. However we have observed positive staining in liver mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is OPA1 supposed to be expressed?

G. Krishna

Verified customer

Asked: 2015-09-25

Answer

From literature, liver does express OPA1. Generally OPA1 expresses in mitochondrion inner membrane. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2015-09-25

Question

I was wanting to use your anti-OPA1 antibody for ICC for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?

C. Edwards

Verified customer

Asked: 2015-01-27

Answer

It shows on the product datasheet, PB9731 anti-OPA1 antibody has been validated for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-01-27

Question

Would PB9731 anti-OPA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Carter

Verified customer

Asked: 2014-07-01

Answer

As indicated on the product datasheet, PB9731 anti-OPA1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-07-01

Order DetailsPrice
PB9731

100μg

$370
PB9731-10ug

10μg sample (liquid)

$99
PB9731-Biotin

100 μg Biotin conjugated

$570
PB9731-Cy3

100 μg Cy3 conjugated

$570
PB9731-Dylight488

100 μg Dylight488 conjugated

$570
PB9731-Dylight550

100 μg Dylight550 conjugated

$570
PB9731-Dylight594

100 μg Dylight594 conjugated

$570
PB9731-FITC

100 μg FITC conjugated

$570
PB9731-HRP

100 μg HRP conjugated

$570
PB9731-APC

100 μg APC conjugated

$670
PB9731-PE

100 μg PE conjugated

$670
PB9731-iFluor647

100 μg iFluor647 conjugated

$670
PB9731-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9731
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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