Product Info Summary
SKU: | PB9731 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-OPA1 Antibody Picoband®
SKU/Catalog Number
PB9731
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-OPA1 Antibody Picoband® catalog # PB9731. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OPA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9731)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9731 is reactive to OPA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80-100 kDa
Calculated molecular weight
111631 MW
Background of OPA1
Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9731 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human MCF-7 whole cell, human PANC-1 whole cell, human PC-3 whole cell, human T47D whole cell, rat brain tissue, mouse brain tissue, mouse heart tissue, mouse NIH/3T3 whole cell
IHC: Mouse Liver tissue, Rat Kidney tissue, Human Lung Cancer tissue
ICC/IF: U20S cell
FCM: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of OPA1 using anti-OPA1 antibody (PB9731).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human T47D whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # PB9731) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100KD. The expected band size for OPA1 is at 80-100KD.
Click image to see more details
Figure 2. IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).
OPA1 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).
OPA1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).
OPA1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Flow Cytometry analysis of U20S cells using anti-OPA1 antibody (PB9731).
Overlay histogram showing U20S cells stained with PB9731 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPA1 Antibody (PB9731,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 6. IF analysis of OPA1 using anti-OPA1 antibody (PB9731).
OPA1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Western blot analysis of OPA1 using anti-OPA1 antibody (PB9731).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # PB9731) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100KD. The expected band size for OPA1 is at 80-100KD.
Protein Target Info & Infographic
Gene/Protein Information For OPA1 (Source: Uniprot.org, NCBI)
Gene Name
OPA1
Full Name
Dynamin-like 120 kDa protein, mitochondrial
Weight
111631 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Dynamin-like 120 kDa protein, mitochondrial;3.6.5.5;Optic atrophy protein 1;Dynamin-like 120 kDa protein, form S1;OPA1;KIAA0567; OPA1 BERHS, MGM1, MTDPS14, NPG, NTG, largeG OPA1 mitochondrial dynamin like GTPase dynamin-like 120 kDa protein, mitochondrial|dynamin-like guanosine triphosphatase|mitochondrial dynamin-like GTPase|optic atrophy 1 (autosomal dominant)|optic atrophy protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OPA1, check out the OPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OPA1 Antibody Picoband® (PB9731)
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Customer Q&As
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17 Customer Q&As for Anti-OPA1 Antibody Picoband®
Question
Our lab used your anti-OPA1 antibody for IF on liver in the past. I am using human, and We intend to use the antibody for IHC-P next. I was wanting to use examining liver as well as corpus callosum in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
I have checked the website and datasheets of our anti-OPA1 antibody and I see that PB9731 has been tested on human in both IF and IHC-P. Thus PB9731 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-27
Question
Is this PB9731 anti-OPA1 antibody reactive to the isotypes of OPA1?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of PB9731 anti-OPA1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
We want using your anti-OPA1 antibody for negative regulation of release of cytochrome c from mitochondria studies. Has this antibody been tested with western blotting on skov whole cell lysate? We would like to see some validation images before ordering.
S. Carter
Verified customer
Asked: 2019-12-09
Answer
We appreciate your inquiry. This PB9731 anti-OPA1 antibody is validated on a549 whole cell lysate, skov whole cell lysate, sw620 whole cell lysate, mouse liver tissue, rat kidney tissue, lung cancer tissue, u20s cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-09
Question
Is a blocking peptide available for product anti-OPA1 antibody (PB9731)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-OPA1 antibody (PB9731). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
Would anti-OPA1 antibody PB9731 work on feline Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Our lab technicians have not tested anti-OPA1 antibody PB9731 on feline. You can run a BLAST between feline and the immunogen sequence of anti-OPA1 antibody PB9731 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-01
Question
My lab would like to test anti-OPA1 antibody PB9731 on mouse brain for research purposes, then I may be interested in using anti-OPA1 antibody PB9731 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The products we sell, including anti-OPA1 antibody PB9731, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-OPA1 antibody PB9731. Let me know if you need anything else.
P. Evans
Verified customer
Asked: 2019-06-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
We have been able to see staining in human liver. What should we do? Is anti-OPA1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
From literature liver does express OPA1. From Uniprot.org, OPA1 is expressed in corpus callosum, brain, liver, among other tissues. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-05-27
Question
Can you help my question with product PB9731, anti-OPA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Brown
Verified customer
Asked: 2019-01-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9731 anti-OPA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-25
Question
We are currently using anti-OPA1 antibody PB9731 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
J. Gonzalez
Verified customer
Asked: 2018-10-19
Answer
The anti-OPA1 antibody (PB9731) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-19
Question
Will anti-OPA1 antibody PB9731 work for ICC with brain?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
According to the expression profile of brain, OPA1 is highly expressed in brain. So, it is likely that anti-OPA1 antibody PB9731 will work for ICC with brain.
Boster Scientific Support
Answered: 2018-08-01
Question
Here is the WB image, lot number and protocol we used for brain using anti-OPA1 antibody PB9731. Please let me know if you require anything else.
O. Lewis
Verified customer
Asked: 2017-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-20
Question
I see that the anti-OPA1 antibody PB9731 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-11
Question
Is there a BSA free version of anti-OPA1 antibody PB9731 available?
G. Krishna
Verified customer
Asked: 2017-07-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-OPA1 antibody PB9731 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-04
Question
Our team were happy with the WB result of your anti-OPA1 antibody. However we have observed positive staining in liver mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is OPA1 supposed to be expressed?
G. Krishna
Verified customer
Asked: 2015-09-25
Answer
From literature, liver does express OPA1. Generally OPA1 expresses in mitochondrion inner membrane. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-09-25
Question
I was wanting to use your anti-OPA1 antibody for ICC for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
C. Edwards
Verified customer
Asked: 2015-01-27
Answer
It shows on the product datasheet, PB9731 anti-OPA1 antibody has been validated for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-27
Question
Would PB9731 anti-OPA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Carter
Verified customer
Asked: 2014-07-01
Answer
As indicated on the product datasheet, PB9731 anti-OPA1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-01