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Product Info Summary
| SKU: | M00508 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-OPA1 Monoclonal Antibody
SKU/Catalog Number
M00508
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-OPA1 Monoclonal Antibody catalog # M00508. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OPA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00508)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACGD-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human OPA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00508 is reactive to OPA1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
85659 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00508 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of OPA1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For OPA1 (Source: Uniprot.org, NCBI)
Gene Name
OPA1
Full Name
Dynamin-like 120 kDa protein, mitochondrial
Weight
85659 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Glucocorticoid receptor;GR;Nuclear receptor subfamily 3 group C member 1;NR3C1;GRL; OPA1 BERHS, MGM1, MTDPS14, NPG, NTG, largeG OPA1 mitochondrial dynamin like GTPase dynamin-like 120 kDa protein, mitochondrial|dynamin-like guanosine triphosphatase|mitochondrial dynamin-like GTPase|optic atrophy 1 (autosomal dominant)|optic atrophy protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OPA1, check out the OPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OPA1 Monoclonal Antibody (M00508)
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No publications found for M00508
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-OPA1 Monoclonal Antibody
Question
Is there a BSA free version of anti-OPA1 Monoclonal antibody M00508 available?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-OPA1 Monoclonal antibody M00508 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-01
Question
We are currently using anti-OPA1 Monoclonal antibody M00508 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-OPA1 Monoclonal antibody (M00508) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
We need using your anti-OPA1 Monoclonal antibody for mitochondrial fission studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
I appreciate your inquiry. This M00508 anti-OPA1 Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-28
Question
We were satisfied with the WB result of your anti-OPA1 Monoclonal antibody. However we have observed positive staining in liver mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is OPA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From what I have seen in literature, liver does express OPA1. Generally OPA1 expresses in mitochondrion inner membrane. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-01-15
Question
We ordered your anti-OPA1 Monoclonal antibody for IF on liver in a previous project. I am using human, and We intend to use the antibody for WB next. My lab would like examining liver as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
I took a look at the website and datasheets of our anti-OPA1 Monoclonal antibody and it appears that M00508 has been validated on human in both IF and WB. Thus M00508 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-03
Question
Is a blocking peptide available for product anti-OPA1 Monoclonal antibody (M00508)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do provide the blocking peptide for product anti-OPA1 Monoclonal antibody (M00508). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-27
Question
Does anti-OPA1 Monoclonal antibody M00508 work for WB with brain?
B. Jha
Verified customer
Asked: 2019-10-01
Answer
According to the expression profile of brain, OPA1 is highly expressed in brain. So, it is likely that anti-OPA1 Monoclonal antibody M00508 will work for WB with brain.
Boster Scientific Support
Answered: 2019-10-01
Question
We want to test anti-OPA1 Monoclonal antibody M00508 on rat brain for research purposes, then I may be interested in using anti-OPA1 Monoclonal antibody M00508 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The products we sell, including anti-OPA1 Monoclonal antibody M00508, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-OPA1 Monoclonal antibody M00508. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-OPA1 Monoclonal antibody M00508 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-03
Question
I have attached the WB image, lot number and protocol we used for brain using anti-OPA1 Monoclonal antibody M00508. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-26
Question
Would M00508 anti-OPA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
You can see on the product datasheet, M00508 anti-OPA1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-10
Question
I was wanting to use your anti-OPA1 Monoclonal antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
W. Huang
Verified customer
Asked: 2018-08-27
Answer
It shows on the product datasheet, M00508 anti-OPA1 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-27
Question
We have observed staining in rat brain. What should we do? Is anti-OPA1 Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
From literature brain does express OPA1. From Uniprot.org, OPA1 is expressed in corpus callosum, brain, liver, among other tissues. Regarding which tissues have OPA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-10-19
Question
Can you help my question with product M00508, anti-OPA1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00508 anti-OPA1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-26
Question
Is this M00508 anti-OPA1 Monoclonal antibody reactive to the isotypes of OPA1?
B. Johnson
Verified customer
Asked: 2013-01-28
Answer
The immunogen of M00508 anti-OPA1 Monoclonal antibody is A synthesized peptide derived from human OPA1 Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-28
