Click image to see more details
Product Info Summary
| SKU: | A07745 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-OLFML2B Olfm1 Antibody
View all Olfactomedin-1/Noelin-1 Antibodies
SKU/Catalog Number
A07745
Size
100ul
Form
Liquid
Description
Boster Bio Anti-OLFML2B Olfm1 Antibody (Catalog# A07745). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OLFML2B Olfm1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07745)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OLFML2B. Immunogen sequence location: 171 - 220
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07745 is reactive to Olfm1 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
55398 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07745 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HUVEC cells, using OLFML2B Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For Olfm1 (Source: Uniprot.org, NCBI)
Gene Name
Olfm1
Full Name
Noelin
Weight
55398 MW
Alternative Names
OLFML2B;Olfactomedin-like protein 2B;Photomedin-2 OLFM1 AMY, NOE1, NOELIN1, OlfA olfactomedin 1 noelin|neuroblastoma protein|neuronal olfactomedin-related ER localized protein|olfactomedin related ER localized protein|pancortin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Olfm1, check out the Olfm1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Olfm1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OLFML2B Olfm1 Antibody (A07745)
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No publications found for A07745
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Customer Q&As
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6 Customer Q&As for Anti-OLFML2B Olfm1 Antibody
Question
We are currently using anti-OLFML2B antibody A07745 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The anti-OLFML2B antibody (A07745) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-OLFML2B antibody A07745. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use your anti-OLFML2B antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
You can see on the product datasheet, A07745 anti-OLFML2B antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-13
Question
I see that the anti-OLFML2B antibody A07745 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-10
Question
My question regarding product A07745, anti-OLFML2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Moore
Verified customer
Asked: 2017-08-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07745 anti-OLFML2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-28
Question
Here is the WB image, lot number and protocol we used for brain using anti-OLFML2B antibody A07745. Please let me know if you require anything else.
V. Anderson
Verified customer
Asked: 2015-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-17
