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Product Info Summary
| SKU: | A00768-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Ogg1 Antibody Picoband®
SKU/Catalog Number
A00768-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ogg1 Antibody Picoband® catalog # A00768-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ogg1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00768-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Ogg1, which shares 89.7% and 87.2% amino acid (aa) sequence identity with mouse and rat Ogg1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00768-1 is reactive to OGG1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
59195 MW
Background of OGG1
8-Oxoguanine glycosylase also known as OGG1 is a DNA glycosylase enzyme that, in humans, is encoded by theOGG1 gene. This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00768-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue, rat thymus tissue, rat liver tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Ogg1 using anti-Ogg1 antibody (A00768-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysate,
Lane 2: rat thymus tissue lysate,
Lane 3: rat liver tissue lysate,
Lane 4: rat testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ogg1 antigen affinity purified polyclonal antibody (Catalog # A00768-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ogg1 at approximately 39KD. The expected band size for Ogg1 is at 39KD.
Protein Target Info & Infographic
Gene/Protein Information For OGG1 (Source: Uniprot.org, NCBI)
Gene Name
OGG1
Full Name
N-glycosylase/DNA lyase
Weight
59195 MW
Superfamily
type-1 OGG1 family
Alternative Names
N-glycosylase/DNA lyase; 8-oxoguanine DNA glycosylase; DNA- (apurinic or apyrimidinic site) lyase; AP lyase; OGG1; MMH; MUTM; OGH1 OGG1 HMMH, HOGG1, MUTM, OGH1 8-oxoguanine DNA glycosylase N-glycosylase/DNA lyase|8-hydroxyguanine DNA glycosylase|AP lyase|DNA-apurinic or apyrimidinic site lyase|OGG1 type 1f
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OGG1, check out the OGG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OGG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ogg1 Antibody Picoband® (A00768-1)
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Customer Q&As
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1 Customer Q&As for Anti-Ogg1 Antibody Picoband®
Question
We are currently using anti-Ogg1 antibody A00768-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
L. Zhao
Verified customer
Asked: 2013-10-15
Answer
The anti-Ogg1 antibody (A00768-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-15
