Anti-APE1/APEX1 Antibody

APE antibody

Boster Bio Anti-APE1/APEX1 Antibody catalog # PA1494. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1494
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-APE1/APEX1 Antibody

View all APE Antibodies

SKU/Catalog Number

PA1494

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-APE1/APEX1 Antibody catalog # PA1494. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APE1/APEX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1494)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human APE1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1494 is reactive to APEX1 in Human, Mouse, Rat

Applications

PA1494 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

35.555kDa

Background of APE

APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For APEX1 (Source: Uniprot.org, NCBI)

Gene Name

APEX1

Full Name

DNA-(apurinic or apyrimidinic site) lyase

Weight

35.555kDa

Superfamily

DNA repair enzymes AP/ExoA family

Alternative Names

AP endonuclease 1; APE; APE1; APE-1; APEAPEX nuclease (multifunctional DNA repair enzyme); APENAP endonuclease class I; APEX deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEX1; Apurinic-apyrimidinic endonuclease 1; APXAP lyase; DNA-(apurinic or apyrimidinic site) lyase; EC 3.1; EC 4.2.99.18; HAP1apurinic/apyrimidinic (abasic) endonuclease; multifunctional DNA repair enzyme; protein REF-1; redox factor 1; Redox factor-1; REF1 apurinic/apyrimidinic exonuclease; REF-1 APEX1 APE, APE1, APEN, APEX, APX, HAP1, REF1 apurinic/apyrimidinic endodeoxyribonuclease 1 DNA-(apurinic or apyrimidinic site) endonuclease|AP endonuclease class I|AP lyase|APEX nuclease (multifunctional DNA repair enzyme) 1|DNA-(apurinic or apyrimidinic site) lyase|apurinic-apyrimidinic endonuclease 1|apurinic/apyrimidinic (abasic) endonuclease|deoxyribonuclease (apurinic or apyrimidinic)|protein REF-1|redox factor-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APEX1, check out the APEX1 Infographic

APEX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APEX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
PA1494

100μg

$370
PA1494-10ug

10μg sample (liquid)

$99
PA1494-Biotin

100 μg Biotin conjugated

$570
PA1494-Cy3

100 μg Cy3 conjugated

$570
PA1494-Dylight488

100 μg Dylight488 conjugated

$570
PA1494-Dylight550

100 μg Dylight550 conjugated

$570
PA1494-Dylight594

100 μg Dylight594 conjugated

$570
PA1494-FITC

100 μg FITC conjugated

$570
PA1494-HRP

100 μg HRP conjugated

$570
PA1494-APC

100 μg APC conjugated

$670
PA1494-PE

100 μg PE conjugated

$670
PA1494-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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