Product Info Summary
SKU: | A06253-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC |
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Product info
Product Name
Anti-OCM Antibody Picoband™
View all Oncomodulin Antibodies
SKU/Catalog Number
A06253-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OCM Antibody Picoband™ catalog # A06253-1. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OCM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06253-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human OCM recombinant protein (Position: M1-S110). Human OCM shares 88.1% and 89% amino acid (aa) sequence identity with mouse and rat OCM, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06253-1 is reactive to Ocm in Human
Applications
A06253-1 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
52588 MW
Background of Oncomodulin
Oncomodulin is a high-affinity calcium ion-binding protein. It belongs to the superfamily of calmodulin proteins, also known as the EF-hand proteins. Oncomodulin is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 2-5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a06253 1 ocm primary antibodies ihc testing 1 a06253 1 ocm primary antibodies ihc testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-1.jpg)
Click image to see more details
Figure 1. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 2 a06253 1 ocm primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 3 a06253 1 ocm primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 4 a06253 1 ocm primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 5 a06253 1 ocm primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-5.jpg)
Click image to see more details
Figure 5. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 6 a06253 1 ocm primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-6.jpg)
Click image to see more details
Figure 6. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a06253 1 ocm primary antibodies ihc testing 7 a06253 1 ocm primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a06253-1-ocm-primary-antibodies-ihc-testing-7.jpg)
Click image to see more details
Figure 7. IHC analysis of OCM using anti-OCM antibody (A06253-1).
OCM was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCM Antibody (A06253-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ocm (Source: Uniprot.org, NCBI)
Gene Name
Ocm
Full Name
Oncomodulin
Weight
52588 MW
Superfamily
parvalbumin family
Alternative Names
OCM; OCM1; OCMN; OM; ONCM; Oncomodulin; Parvalbumin beta Ocm|oncomodulin|oncomodulin|OM|parvalbumin beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ocm, check out the Ocm Infographic
![Ocm infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ocm: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OCM Antibody Picoband™ (A06253-1)
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