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Product Info Summary
| SKU: | A02456-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-OAT1/SLC22A6 Antibody Picoband®
SKU/Catalog Number
A02456-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-OAT1/SLC22A6 Antibody Picoband® catalog # A02456-1. Tested in WB,FCM applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OAT1/SLC22A6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02456-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human OAT1/SLC22A6 recombinant protein (Position: R286-L563). Human SLC22A6 shares 82% and 83.8% amino acid (aa) sequence identity with mouse and rat SLC22A6, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02456-1 is reactive to SLC22A6 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
62 kDa
Background of SLC22A6
The organic anion transporter 1 (OAT1) also known as solute carrier family 22 member 6 (SLC22A6) is a protein that in humans is encoded by the SLC22A6 gene. The protein encoded by this gene is involved in the sodium-dependent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and may be localized to the basolateral membrane. Four transcript variants encoding four different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02456-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human placenta tissue
FCM: THP-1 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OAT1/SLC22A6 using anti-OAT1/SLC22A6 antibody (A02456-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT1/SLC22A6 antigen affinity purified polyclonal antibody (Catalog # A02456-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT1/SLC22A6 at approximately 70 kDa. The expected band size for OAT1/SLC22A6 is at 62 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-OAT1/SLC22A6 antibody (A02456-1).
Overlay histogram showing THP-1 cells stained with A02456-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OAT1/SLC22A6 Antibody (A02456-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SLC22A6 (Source: Uniprot.org, NCBI)
Gene Name
SLC22A6
Full Name
Solute carrier family 22 member 6
Weight
62 kDa
Superfamily
major facilitator (TC 2.A.1) superfamily
Alternative Names
hOAT1; hPAHT; hROAT1; MGC45260; OAT1; OAT1FLJ55736; Organic anion transporter 1; PAH transporter; PAHT; PAHTHOAT1; para-aminohippurate transporter; Renal organic anion transporter 1; ROAT1; SLC22A6; solute carrier family 22 (organic anion transporter), member 6; solute carrier family 22 member 6 SLC22A6 HOAT1, OAT1, PAHT, ROAT1 solute carrier family 22 member 6 solute carrier family 22 member 6|PAH transporter|hPAHT|hROAT1|organic anion transporter 1|para-aminohippurate transporter|renal organic anion transporter 1|solute carrier family 22 (organic anion transporter), member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC22A6, check out the SLC22A6 Infographic
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In this infographic, you will see the following information for SLC22A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OAT1/SLC22A6 Antibody Picoband® (A02456-1)
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