Click image to see more details
Product Info Summary
| SKU: | PB9302 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NUP98 Antibody Picoband®
SKU/Catalog Number
PB9302
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUP98 Antibody Picoband® catalog # PB9302. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NUP98 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9302)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUP98 recombinant protein (Position: H549-F880). Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9302 is reactive to NUP98 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
98 kDa
Calculated molecular weight
197579 MW
Background of NUP98
Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene. This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC. NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis. It also plays roles in gene expression, mitotic checkpoint, and pathogenesis. Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates. In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9302 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human COLO320 whole cell, human HepG2 whole cell, human Hacat whole cell, monkey COS-7 whole cell, rat NRK whole cell, mouse lung tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NUP98 using anti-NUP98 antibody (PB9302).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human Hacat whole cell lysates,
Lane 7: monkey COS-7 whole cell lysates,
Lane 8: rat NRK whole cell lysates,
Lane 9: mouse lung tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP98 antigen affinity purified polyclonal antibody (Catalog # PB9302) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP98 at approximately 98 kDa. The expected band size for NUP98 is at 198 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NUP98 (Source: Uniprot.org, NCBI)
Gene Name
NUP98
Full Name
Nuclear pore complex protein Nup98-Nup96
Weight
197579 MW
Superfamily
nucleoporin GLFG family
Alternative Names
Nuclear pore complex protein Nup98-Nup96;Nuclear pore complex protein Nup98;98 kDa nucleoporin;Nucleoporin Nup98;Nup98;Nuclear pore complex protein Nup96;96 kDa nucleoporin;Nucleoporin Nup96;Nup96;NUP98;ADAR2; NUP98 ADIR2, NUP196, NUP96, Nup98-96 nucleoporin 98 and 96 precursor nuclear pore complex protein Nup98-Nup96|nuclear pore complex protein Nup98|GLFG-repeat containing nucleoporin|NUP98/PHF23 fusion 2 protein|Nup98-Nup96|nucleoporin 96|nucleoporin 98kD|nucleoporin 98kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUP98, check out the NUP98 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUP98: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUP98 Antibody Picoband® (PB9302)
Hello CJ!
No publications found for PB9302
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NUP98 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NUP98 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-NUP98 Antibody Picoband®
Question
Would anti-NUP98 antibody PB9302 work for WB with lung carcinoma?
J. Moore
Verified customer
Asked: 2020-02-10
Answer
According to the expression profile of lung carcinoma, NUP98 is highly expressed in lung carcinoma. So, it is likely that anti-NUP98 antibody PB9302 will work for WB with lung carcinoma.
Boster Scientific Support
Answered: 2020-02-10
Question
See attached the WB image, lot number and protocol we used for lung carcinoma using anti-NUP98 antibody PB9302. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
Do you have a BSA free version of anti-NUP98 antibody PB9302 available?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NUP98 antibody PB9302 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-21
Question
We are currently using anti-NUP98 antibody PB9302 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
The anti-NUP98 antibody (PB9302) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-12
