Product Info Summary
SKU: | PB9301 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NUMB Antibody Picoband™
SKU/Catalog Number
PB9301
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUMB Antibody Picoband™ catalog # PB9301. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NUMB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9301)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9301 is reactive to NUMB in Human, Monkey, Mouse, Rat
Applications
PB9301 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
71 kDa
Calculated molecular weight
70804 MW
Background of NUMB
Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What’s more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
![pb9301 numb primary antibodies wb testing 1 pb9301 numb primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/p/b/pb9301-numb-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (PB9301).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # PB9301) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 71 kDa. The expected band size for NUMB is at 71 kDa.
![pb9301 numb primary antibodies ihc testing 2 pb9301 numb primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/p/b/pb9301-numb-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of NUMB using anti-NUMB antibody (PB9301).
NUMB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUMB Antibody (PB9301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![pb9301 numb primary antibodies ihc testing 3 pb9301 numb primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/p/b/pb9301-numb-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of NUMB using anti-NUMB antibody (PB9301).
NUMB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUMB Antibody (PB9301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![pb9301 numb primary antibodies fcm testing 4 pb9301 numb primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/p/b/pb9301-numb-primary-antibodies-fcm-testing-4.jpg)
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Figure 4. Flow Cytometry analysis of U87 cells using anti-NUMB antibody (PB9301).
Overlay histogram showing U87 cells stained with PB9301 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMB Antibody (PB9301, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NUMB (Source: Uniprot.org, NCBI)
Gene Name
NUMB
Full Name
Protein numb homolog
Weight
70804 MW
Alternative Names
h-Numb; mnb; m-numb; numb (Drosophila) homolog; numb homolog (Drosophila); Numb; protein numb homolog; Protein S171; S171 NUMB C14orf41, S171, c14_5527 NUMB endocytic adaptor protein protein numb homolog|h-Numb|numb homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUMB, check out the NUMB Infographic
![NUMB infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUMB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUMB Antibody Picoband™ (PB9301)
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Customer Q&As
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7 Customer Q&As for Anti-NUMB Antibody Picoband™
Question
I have a question about product PB9301, anti-NUMB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9301 anti-NUMB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-13
Question
I see that the anti-NUMB antibody PB9301 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-22
Question
Is this PB9301 anti-NUMB antibody reactive to the isotypes of NUMB?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of PB9301 anti-NUMB antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB(194-220aa SFRVTTATEQAEREEIMKQMQDAKKAE), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
Do you have a BSA free version of anti-NUMB antibody PB9301 available?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NUMB antibody PB9301 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-01
Question
Is a blocking peptide available for product anti-NUMB antibody (PB9301)?
G. Collins
Verified customer
Asked: 2018-10-11
Answer
We do provide the blocking peptide for product anti-NUMB antibody (PB9301). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-11
Question
Will anti-NUMB antibody PB9301 work on goat WB with placenta?
E. Anderson
Verified customer
Asked: 2013-10-31
Answer
Our lab technicians have not validated anti-NUMB antibody PB9301 on goat. You can run a BLAST between goat and the immunogen sequence of anti-NUMB antibody PB9301 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-31
Question
We are currently using anti-NUMB antibody PB9301 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
A. Wu
Verified customer
Asked: 2013-05-17
Answer
The anti-NUMB antibody (PB9301) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-17