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Product Info Summary
| SKU: | A10137-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NUFIP1 Antibody Picoband®
SKU/Catalog Number
A10137-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUFIP1 Antibody Picoband® catalog # A10137-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUFIP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10137-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUFIP1 recombinant protein (Position: Q66-D481).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A10137-1 is reactive to NUFIP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
41409 MW
Background of NUFIP1
Nuclear fragile X mental retardation-interacting protein 1 is a protein that in humans is encoded by the NUFIP1 gene. This gene encodes a nuclear RNA binding protein that contains a C2H2 zinc finger motif and a nuclear localization signal. This protein is associated with the nuclear matrix in perichromatin fibrils and, in neurons, localizes to the cytoplasm in association with endoplasmic reticulum ribosomes. This protein interacts with the fragile X mental retardation protein (FMRP), the tumor suppressor protein BRCA1, upregulates RNA polymerase II transcription, and is involved in box C/D snoRNP biogenesis. A pseudogene of this gene resides on chromosome 6q12.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10137-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human A375 whole cell, human Caco-2 whole cell, human HEL whole cell, human 293T whole cell, human HepG2 whole cell, human SH-SY5Y whole cell
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NUFIP1 using anti-NUFIP1 antibody (A10137-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human 293T whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUFIP1 antigen affinity purified polyclonal antibody (Catalog # A10137-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUFIP1 at approximately 75 kDa. The expected band size for NUFIP1 is at 75 kDa.
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Figure 2. IF analysis of NUFIP1 using anti-NUFIP1 antibody (A10137-1) and anti-Beta Tubulin antibody (M01857-3).
NUFIP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUFIP1 Antibody (A10137-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For NUFIP1 (Source: Uniprot.org, NCBI)
Gene Name
NUFIP1
Full Name
Nuclear fragile X mental retardation-interacting protein 1
Weight
41409 MW
Alternative Names
Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; NUFIP1 NUFIP, Rsa1, bA540M5.1 nuclear FMR1 interacting protein 1 nuclear fragile X mental retardation-interacting protein 1|NUFIP1, FMR1 interacting protein 1|nuclear FMRP-interacting protein 1|nuclear fragile X mental retardation protein interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUFIP1, check out the NUFIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUFIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUFIP1 Antibody Picoband® (A10137-1)
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