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Product Info Summary
| SKU: | A08538-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NUBP1 Antibody Picoband®
SKU/Catalog Number
A08538-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUBP1 Antibody Picoband® catalog # A08538-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08538-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUBP1 recombinant protein (Position: M1-Q311).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08538-1 is reactive to NUBP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.534kDa
Background of NUBP1
NUBP1 also known as NBP as is expressed in several tissues with the highest being in the lung and testis. NUBP1 appears to interact with actin and the cytoskeleton and is required for centrosome duplication but it is also need for proper synthesis of iron sulfur proteins and the protein also binds ATP. NUBP1 has roles in cell growth, cellular iron homeostasis, centrosome localization, iron-sulfur cluster assembly, and apolipoprotein modification. NUBP1 is localized to the cytoplasm. Interestingly too, recent research indicates that mutations in NUBP1 in mice lead defects in lung development and eye cataracts, many of which are traced back to NUBP1 role in regulating centrosome dynamics and microtubule organization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08538-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human A549 whole cell, human RT4 whole cell, human U251 whole cell, human Colo320 whole cell, human HepG2 whole cell, human U20S whole cell, human 293T whole cell
ICC/IF: HELA cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NUBP1 using anti-NUBP1 antibody (A08538-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: human Colo320 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human U20S whole cell lysates,
Lane 8: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBP1 antigen affinity purified polyclonal antibody (Catalog # A08538-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBP1 at approximately 35 kDa. The expected band size for NUBP1 is at 35 kDa.
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Figure 2. IF analysis of NUBP1 using anti-NUBP1 antibody (A08538-1).
NUBP1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUBP1 Antibody (A08538-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of K562 cells using anti-NUBP1 antibody (A08538-1).
Overlay histogram showing K562 cells stained with A08538-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUBP1 Antibody (A08538-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NUBP1 (Source: Uniprot.org, NCBI)
Gene Name
NUBP1
Full Name
Cytosolic Fe-S cluster assembly factor NUBP1
Weight
34.534kDa
Superfamily
Mrp/NBP35 ATP-binding proteins family
Alternative Names
PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006 NUBP1 CIAO5, NBP, NBP1, NBP35 nucleotide binding protein 1 cytosolic Fe-S cluster assembly factor NUBP1|NBP 1|nucleotide binding protein (e.coli MinD like)|nucleotide binding protein 1 (E.coli MinD like)|nucleotide binding protein 1 (MinD homolog, E. coli)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUBP1, check out the NUBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUBP1 Antibody Picoband® (A08538-1)
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