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Product Info Summary
| SKU: | A06935-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NT-4/NTF4 Antibody Picoband®
SKU/Catalog Number
A06935-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NT-4/NTF4 Antibody Picoband® catalog # A06935-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NT-4/NTF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06935-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NT-4/NTF4, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat NT-4/NTF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06935-3 is reactive to NTF4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
87148 MW
Background of NT-4
Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06935-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HEK293 whole cell, human SW620 whole cell, human Hela whole cell, rat RH-35 whole cell, mouse spleen tissue, mouse thymus tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NT-4/NTF4 using anti-NT-4/NTF4 antibody (A06935-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT-4/NTF4 antigen affinity purified polyclonal antibody (Catalog # A06935-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT-4/NTF4 at approximately 24KD. The expected band size for NT-4/NTF4 is at 24KD.
Protein Target Info & Infographic
Gene/Protein Information For NTF4 (Source: Uniprot.org, NCBI)
Gene Name
NTF4
Full Name
Neurotrophin-4
Weight
87148 MW
Superfamily
NGF-beta family
Alternative Names
Interleukin-34; IL-34; IL34; C16orf77 NTF4 GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5 neurotrophin 4 neurotrophin-4|neurotrophic factor 4|neurotrophic factor 5|neurotrophin 5 (neurotrophin 4/5)|neurotrophin-5|neutrophic factor 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NTF4, check out the NTF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NTF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NT-4/NTF4 Antibody Picoband® (A06935-3)
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