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Product Info Summary
| SKU: | M01332 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody
View all LRH-1/NR5A2 Antibodies
SKU/Catalog Number
M01332
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody catalog # M01332. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01332)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABHO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NR5A2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01332 is reactive to NR5A2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
105 kDa
Calculated molecular weight
61331 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01332 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis of NR5A2 expression in MCF7 cell lysate.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For NR5A2 (Source: Uniprot.org, NCBI)
Gene Name
NR5A2
Full Name
Nuclear receptor subfamily 5 group A member 2
Weight
61331 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Nuclear receptor subfamily 5 group A member 2;Alpha-1-fetoprotein transcription factor;B1-binding factor;hB1F;CYP7A promoter-binding factor;Hepatocytic transcription factor;Liver receptor homolog 1;LRH-1;NR5A2;B1F, CPF, FTF; NR5A2 B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2 nuclear receptor subfamily 5 group A member 2 nuclear receptor subfamily 5 group A member 2|CYP7A promoter-binding factor|b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus|fetoprotein-alpha 1 (AFP) transcription factor|hepatocytic transcription factor hB1F-3|liver nuclear receptor homolog-1 variant 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR5A2, check out the NR5A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody (M01332)
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No publications found for M01332
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Customer Q&As
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5 Customer Q&As for Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
I have attached the WB image, lot number and protocol we used for liver using anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
I have a question about product M01332, anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01332 anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
You can see on the product datasheet, M01332 anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-01
Question
Does anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
Our lab technicians have not validated anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-05
