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Product Info Summary
| SKU: | M01521 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NR0B1 / DAX1 Monoclonal Antibody
View all DAX1/NR0B1 Antibodies
SKU/Catalog Number
M01521
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NR0B1 / DAX1 Monoclonal Antibody catalog # M01521. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR0B1 / DAX1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01521)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AECC-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NR0B1 / Dax1 Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01521 is reactive to NR0B1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
54002 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01521 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of NR0B1 / Dax1 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NR0B1 (Source: Uniprot.org, NCBI)
Gene Name
NR0B1
Full Name
Nuclear receptor subfamily 0 group B member 1
Weight
54002 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; NR0B1 AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 nuclear receptor subfamily 0 group B member 1 nuclear receptor subfamily 0 group B member 1|DSS-AHC critical region on the X chromosome protein 1|nuclear hormone receptor|nuclear receptor DAX-1|truncated nuclear receptor subfamily 0 group B member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR0B1, check out the NR0B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR0B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR0B1 / DAX1 Monoclonal Antibody (M01521)
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No publications found for M01521
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Customer Q&As
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6 Customer Q&As for Anti-NR0B1 / DAX1 Monoclonal Antibody
Question
I see that the anti-NR0B1 / DAX1 Monoclonal antibody M01521 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-NR0B1 / DAX1 Monoclonal antibody M01521. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
See below the WB image, lot number and protocol we used for lung using anti-NR0B1 / DAX1 Monoclonal antibody M01521. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-04
Question
Is this M01521 anti-NR0B1 / DAX1 Monoclonal antibody reactive to the isotypes of NR0B1?
Verified Customer
Verified customer
Asked: 2019-03-13
Answer
The immunogen of M01521 anti-NR0B1 / DAX1 Monoclonal antibody is A synthesized peptide derived from human NR0B1 / Dax1 Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-13
Question
Do you have a BSA free version of anti-NR0B1 / DAX1 Monoclonal antibody M01521 available?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NR0B1 / DAX1 Monoclonal antibody M01521 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-21
Question
We are currently using anti-NR0B1 / DAX1 Monoclonal antibody M01521 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
The anti-NR0B1 / DAX1 Monoclonal antibody (M01521) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-31
