Anti-NR0B1 / DAX1 Monoclonal Antibody

DAX1/NR0B1 antibody

Boster Bio Anti-NR0B1 / DAX1 Monoclonal Antibody catalog # M01521. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M01521
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-NR0B1 / DAX1 Monoclonal Antibody

View all DAX1/NR0B1 Antibodies

SKU/Catalog Number

M01521

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NR0B1 / DAX1 Monoclonal Antibody catalog # M01521. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NR0B1 / DAX1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01521)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AECC-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NR0B1 / Dax1 Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01521 is reactive to NR0B1 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

43 kDa

Calculated molecular weight

54002 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M01521 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50

Positive Control

WB: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For NR0B1 (Source: Uniprot.org, NCBI)

Gene Name

NR0B1

Full Name

Nuclear receptor subfamily 0 group B member 1

Weight

54002 MW

Superfamily

nuclear hormone receptor family

Alternative Names

Macrophage metalloelastase;MME;3.4.24.65;Macrophage elastase;ME;hME;Matrix metalloproteinase-12;MMP-12;MMP12;HME; NR0B1 AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 nuclear receptor subfamily 0 group B member 1 nuclear receptor subfamily 0 group B member 1|DSS-AHC critical region on the X chromosome protein 1|nuclear hormone receptor|nuclear receptor DAX-1|truncated nuclear receptor subfamily 0 group B member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NR0B1, check out the NR0B1 Infographic

NR0B1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NR0B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M01521

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-NR0B1 / DAX1 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-NR0B1 / DAX1 Monoclonal Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-NR0B1 / DAX1 Monoclonal Antibody

Question

I see that the anti-NR0B1 / DAX1 Monoclonal antibody M01521 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-05-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-NR0B1 / DAX1 Monoclonal antibody M01521. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-11

Question

See below the WB image, lot number and protocol we used for lung using anti-NR0B1 / DAX1 Monoclonal antibody M01521. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-04

Question

Is this M01521 anti-NR0B1 / DAX1 Monoclonal antibody reactive to the isotypes of NR0B1?

Verified Customer

Verified customer

Asked: 2019-03-13

Answer

The immunogen of M01521 anti-NR0B1 / DAX1 Monoclonal antibody is A synthesized peptide derived from human NR0B1 / Dax1 Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-13

Question

Do you have a BSA free version of anti-NR0B1 / DAX1 Monoclonal antibody M01521 available?

Verified Customer

Verified customer

Asked: 2019-01-21

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NR0B1 / DAX1 Monoclonal antibody M01521 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-21

Question

We are currently using anti-NR0B1 / DAX1 Monoclonal antibody M01521 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

The anti-NR0B1 / DAX1 Monoclonal antibody (M01521) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-31

Order DetailsPrice
M01521

100μL

$370
M01521-30ul

30μL sample

$99
M01521-carrier-free-1ml

Carrier Free

$2590

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
M01521
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.