Anti-Podocin NPHS2 Antibody Picoband®

Product Name

Anti-Podocin NPHS2 Antibody Picoband®

View all Podocin/NPHS2 Antibodies

SKU/Catalog Number

PA1322

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Podocin NPHS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1322)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NPHS2, different from the mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1322 is reactive to NPHS2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

42201 MW

Background of Podocin/NPHS2

Podocin (PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al. (2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1322 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Positive Control

WB: Rat Kidney Tissue

Validation Images & Assay Conditions

Gene/Protein Information For NPHS2 (Source: Uniprot.org, NCBI)

Alternative Names

Podocin;NPHS2; NPHS2 PDCN, SRN1 NPHS2 stomatin family member, podocin podocin|NPHS2, podocin|nephrosis 2, idiopathic, steroid-resistant (podocin)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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