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WB: Rat Kidney Tissue Lysate
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WB: Rat Kidney Tissue Lysate
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WB: Rat Kidney Tissue Lysate
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Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
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Key=description, value=Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Podocin (PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al. (2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.
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Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
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Key=immunogen, value=A synthetic peptide corresponding to a sequence in the middle region of human NPHS2, different from the mouse sequence by one amino acid.
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Anti-Podocin NPHS2 Antibody Picoband®

Podocin/NPHS2 antibody

Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 7 publication(s).

Product Info Summary

SKU: PA1322
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Podocin NPHS2 Antibody Picoband®

View all Podocin/NPHS2 Antibodies

SKU/Catalog Number

PA1322

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Podocin NPHS2 Antibody catalog # PA1322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Podocin NPHS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1322)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NPHS2, different from the mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1322 is reactive to NPHS2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

42201 MW

Background of Podocin/NPHS2

Podocin (PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al. (2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1322 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Positive Control

WB: Rat Kidney Tissue

Validation Images & Assay Conditions

Gene/Protein Information For NPHS2 (Source: Uniprot.org, NCBI)

Gene Name

NPHS2

Full Name

Podocin

Weight

42201 MW

Superfamily

band 7/mec-2 family

Alternative Names

Podocin;NPHS2; NPHS2 PDCN, SRN1 NPHS2 stomatin family member, podocin podocin|NPHS2, podocin|nephrosis 2, idiopathic, steroid-resistant (podocin)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NPHS2, check out the NPHS2 Infographic

NPHS2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NPHS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1322 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qiu Y,Zhou J,Zhang H,Zhou H,Tang H,Lei C,Ye C,You C,Chen Y,Wang Y,Xiong J,Su H,Yao G,Zhang C.Rhodojaponin II attenuates kidney injury by regulating TGF-β1/Smad pathway in mice with adriamycin nephropathy.J Ethnopharmacol.2019 Oct 28;243:112078.doi:10.1016/j.jep.2019.112078.Epub 2019 Jul 10.PMID: 31301369.
Species: Mouse
PA1322 usage in article: APP:WB, SAMPLE:RENAL TISSUE, DILUTION:1:200

Zhang X,Zhang L,Chen Z,Li S,Che B, Wang N,Chen J,Xu C,Wei C.Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.Int J Mol Med.2021 Mar;47(3):27.doi:10.3892/ijmm.2021.4860.Epub 2021 Feb 4.PMID:33537831.
Species: Rat
PA1322 usage in article: APP:WB, SAMPLE:RENAL TISSUE AND PODOCYTES, DILUTION:1:400

Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
PA1322 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse

Effect of simvastatin on the expression of nephrin, podocin, and vascular endothelial growth factor (VEGF) in podocytes of diabetic rat

Hao L, Pan Ms, Zheng Y, Wang Rf. Exp Ther Med. 2014 Jun;7(6):1465-1470. Epub 2014 Apr 7. Effect Of Cordyceps Sinensis And Tripterygium Wilfordii Polyglycosidium On Podocytes In Rats With Diabetic Nephropathy.

Qu Y, Du E, Zhang Y, Li S, Han R, Qiu M. Nephrourol Mon. 2012 Spring;4(2):466-9. Doi: 10.5812/Numonthly.2124. Epub 2012 Mar 1. Changes In The Expression Of Bone Morphogenetic Protein 7 And Tamm- Horsfall Protein In The Early Stages Of Diabetic Nep...

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7 Customer Q&As for Anti-Podocin NPHS2 Antibody Picoband®

Question

Is a blocking peptide available for product anti-NPHS2 antibody (PA1322)?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

We do provide the blocking peptide for product anti-NPHS2 antibody (PA1322). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-01

Question

I see that the anti-NPHS2 antibody PA1322 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-13

Question

I was wanting to use your anti-NPHS2 antibody for WB for human renal glomerulus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human renal glomerulus identification?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

It shows on the product datasheet, PA1322 anti-NPHS2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human renal glomerulus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-14

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal glomerulus using anti-NPHS2 antibody PA1322. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this PA1322 anti-NPHS2 antibody reactive to the isotypes of NPHS2?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

The immunogen of PA1322 anti-NPHS2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-17

Question

Will PA1322 anti-NPHS2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-02-16

Answer

You can see on the product datasheet, PA1322 anti-NPHS2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-16

Question

I have a question about product PA1322, anti-NPHS2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1322 anti-NPHS2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-15

Order DetailsPrice
PA1322

100μg

$370
PA1322-10ug

10μg sample (liquid)

$99
PA1322-Biotin

100 μg Biotin conjugated

$570
PA1322-Cy3

100 μg Cy3 conjugated

$570
PA1322-Dylight488

100 μg Dylight488 conjugated

$570
PA1322-Dylight550

100 μg Dylight550 conjugated

$570
PA1322-Dylight594

100 μg Dylight594 conjugated

$570
PA1322-FITC

100 μg FITC conjugated

$570
PA1322-HRP

100 μg HRP conjugated

$570
PA1322-APC

100 μg APC conjugated

$670
PA1322-PE

100 μg PE conjugated

$670
PA1322-iFluor647

100 μg iFluor647 conjugated

$670
PA1322-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1322
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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