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Product Info Summary
| SKU: | PA1215 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Notch1 Antibody Picoband®
SKU/Catalog Number
PA1215
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Notch1 Antibody catalog # PA1215. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Notch1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1215)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Notch1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1215 is reactive to NOTCH1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
272 kDa
Calculated molecular weight
272505 MW
Background of NOTCH1
Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1215 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell, HELA Whole Cell, JURKAT Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
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Anti-Notch1 antibody, PA1215, Western blotting
All lanes: Anti Notch1 (PA1215) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 272KD
Observed bind size: 272KD
Protein Target Info & Infographic
Gene/Protein Information For NOTCH1 (Source: Uniprot.org, NCBI)
Gene Name
NOTCH1
Full Name
Neurogenic locus notch homolog protein 1
Weight
272505 MW
Superfamily
NOTCH family
Alternative Names
Neurogenic locus notch homolog protein 1;Notch 1;hN1;Translocation-associated notch protein TAN-1;Notch 1 extracellular truncation;NEXT;Notch 1 intracellular domain;NICD;NOTCH1;TAN1; NOTCH1 AOS5, AOVD1, TAN1, hN1 notch receptor 1 neurogenic locus notch homolog protein 1|Notch homolog 1, translocation-associated|notch 1|translocation-associated notch protein TAN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOTCH1, check out the NOTCH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOTCH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Notch1 Antibody Picoband® (PA1215)
Hello CJ!
PA1215 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mi L, Chen Y, Zheng X, Li Y, Zhang Q, Mo D, Yang G. J Cell Biochem. 2015 Jul;116(7):1195-204. Doi: 10.1002/Jcb.25065. Microrna-139-5P Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch And Irs1/Pi3K/Akt Insulin Signaling Pathways.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Notch1 Antibody Picoband®
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Notch1 antibody PA1215. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-06
Question
Do you have a BSA free version of anti-Notch1 antibody PA1215 available?
E. Anderson
Verified customer
Asked: 2020-04-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Notch1 antibody PA1215 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-08
Question
My question regarding product PA1215, anti-Notch1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1215 anti-Notch1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
Does anti-Notch1 antibody PA1215 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
According to the expression profile of cervix carcinoma, NOTCH1 is highly expressed in cervix carcinoma. So, it is likely that anti-Notch1 antibody PA1215 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-Notch1 antibody PA1215 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-18
Question
We have seen staining in human cervix carcinoma. Any tips? Is anti-Notch1 antibody supposed to stain cervix carcinoma positively?
J. Brown
Verified customer
Asked: 2019-08-30
Answer
From what I have seen in literature cervix carcinoma does express NOTCH1. From what I have seen in Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2019-08-30
Question
My question regards to test anti-Notch1 antibody PA1215 on human cervix carcinoma for research purposes, then I may be interested in using anti-Notch1 antibody PA1215 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The products we sell, including anti-Notch1 antibody PA1215, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-28
Question
Is a blocking peptide available for product anti-Notch1 antibody (PA1215)?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
We do provide the blocking peptide for product anti-Notch1 antibody (PA1215). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-27
Question
Is this PA1215 anti-Notch1 antibody reactive to the isotypes of NOTCH1?
H. Johnson
Verified customer
Asked: 2018-03-21
Answer
The immunogen of PA1215 anti-Notch1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Notch1(2487-2506aa QHSYSSPVDNTPSHQLQVPE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Notch1 antibody PA1215. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-29
Question
We are currently using anti-Notch1 antibody PA1215 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
V. Banerjee
Verified customer
Asked: 2017-08-25
Answer
The anti-Notch1 antibody (PA1215) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-25
Question
We were happy with the WB result of your anti-Notch1 antibody. However we have been able to see positive staining in cervix carcinoma notch 1 intracellular domain: nucleus using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?
L. Yang
Verified customer
Asked: 2014-12-24
Answer
According to literature, cervix carcinoma does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2014-12-24
Question
Does anti-Notch1 antibody PA1215 work on feline WB with brain?
A. Jones
Verified customer
Asked: 2014-08-14
Answer
Our lab technicians have not tested anti-Notch1 antibody PA1215 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Notch1 antibody PA1215 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-14
Question
I was wanting to use your anti-Notch1 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
J. Zhao
Verified customer
Asked: 2014-02-11
Answer
It shows on the product datasheet, PA1215 anti-Notch1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-02-11
Question
I am interested in using your anti-Notch1 antibody for defective lfng causes scdo3 studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.
K. Collins
Verified customer
Asked: 2014-01-09
Answer
We appreciate your inquiry. This PA1215 anti-Notch1 antibody is validated on hela whole cell lysate, jurkat whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-01-09
Question
Does PA1215 anti-Notch1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Li
Verified customer
Asked: 2013-12-09
Answer
It shows on the product datasheet, PA1215 anti-Notch1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-09
