Anti-NOB1 Antibody Picoband™

Product Name

Anti-NOB1 Antibody Picoband™

View all NOB1 Antibodies

SKU/Catalog Number

A03504-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOB1 Antibody Picoband™ catalog # A03504-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NOB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03504-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NOB1 recombinant protein (Position: M1-D389).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03504-1 is reactive to NOB1 in Human, Mouse, Rat

Applications

A03504-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

Background of NOB1

RNA-binding protein NOB1 is a protein that in humans is encoded by the NOB1 gene. In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NOB1 using anti-NOB1 antibody (A03504-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOB1 antigen affinity purified polyclonal antibody (Catalog # A03504-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOB1 at approximately 47 kDa. The expected band size for NOB1 is at 47 kDa.

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Figure 2. IHC analysis of NOB1 using anti-NOB1 antibody (A03504-1).
NOB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOB1 Antibody (A03504-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of NOB1 using anti-NOB1 antibody (A03504-1).
NOB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOB1 Antibody (A03504-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For NOB1 (Source: Uniprot.org, NCBI)

Alternative Names

adenocarcinoma antigen recognized by T lymphocytes 4; ART4; ART-4; MST158; nin one binding protein; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); NOB1PPSMD8BP1; Phosphorylation regulatory protein HP-10; Protein ART-4; PSMD8 binding protein 1; RNA-binding protein NOB1 NOB1 ART-4, MST158, MSTP158P, PSMD8BP1, NOB1 NIN1 (RPN12) binding protein 1 homolog RNA-binding protein NOB1|NIN1/PSMD8 binding protein 1 homolog|PSMD8 binding protein 1|adenocarcinoma antigen recognized by T lymphocytes 4|nin one binding protein|phosphorylation regulatory protein HP-10|protein ART-4

For more info on NOB1, check out the NOB1 Infographic

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In this infographic, you will see the following information for NOB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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