Product Info Summary
SKU: | A14119-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NLRP9 Antibody Picoband®
SKU/Catalog Number
A14119-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NLRP9 Antibody Picoband® catalog # A14119-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NLRP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14119-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NLRP9 recombinant protein (Position: H118-H746).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14119-1 is reactive to NLRP9 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
113 kDa
Calculated molecular weight
64099 MW
Background of NLRP9
The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14119-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human 293T whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NLRP9 using anti-NLRP9 antibody (A14119-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP9 antigen affinity purified polyclonal antibody (Catalog # A14119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP9 at approximately 113 kDa. The expected band size for NLRP9 is at 113 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NLRP9 (Source: Uniprot.org, NCBI)
Gene Name
NLRP9
Full Name
NACHT, LRR and PYD domains-containing protein 9
Weight
64099 MW
Superfamily
NLRP family
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47; NLRP9 CLR19.1, NALP9, NOD6, PAN12 NLR family pyrin domain containing 9 NACHT, LRR and PYD domains-containing protein 9|NACHT, LRR and PYD containing protein 9|NACHT, leucine rich repeat and PYD containing 9|PYRIN and NACHT-containing protein 12|nucleotide-binding oligomerization domain protein 6|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NLRP9, check out the NLRP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NLRP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NLRP9 Antibody Picoband® (A14119-1)
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