Anti-NKX6.3 Antibody

NKX6.3 antibody

Boster Bio Anti-NKX6.3 Antibody (Catalog# A30461). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Product Info Summary

SKU: A30461
Size: 100ul
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-NKX6.3 Antibody

View all NKX6.3 Antibodies

SKU/Catalog Number

A30461

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NKX6.3 Antibody (Catalog# A30461). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NKX6.3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30461)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human NKX6.3. Immunogen sequence location: 161-210

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30461 is reactive to Nkx6-3 in Human, Monkey, Mouse

Applications

A30461 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

28948 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For Nkx6-3 (Source: Uniprot.org, NCBI)

Gene Name

Nkx6-3

Full Name

Homeobox protein Nkx-6.3

Weight

28948 MW

Alternative Names

FLJ25169; homeobox protein Nkx-6.3; NK6 homeobox 3; NK6 transcription factor related, locus 3 (Drosophila); NK6 transcription factor related, locus 3; NKX6.3 Nkx6-3|9130417I07Rik, Nkx6., Nkx6.3|NK6 homeobox 3|homeobox protein Nkx-6.3|NK6 transcription factor related, locus 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Nkx6-3, check out the Nkx6-3 Infographic

Nkx6-3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Nkx6-3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-NKX6.3 Antibody

Question

We are currently using anti-NKX6.3 antibody A30461 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

The anti-NKX6.3 antibody (A30461) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-05-07

Question

I was wanting to use your anti-NKX6.3 antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2017-11-17

Answer

It shows on the product datasheet, A30461 anti-NKX6.3 antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-17

Question

My question regards to test anti-NKX6.3 antibody A30461 on human brain for research purposes, then I may be interested in using anti-NKX6.3 antibody A30461 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Krishna

Verified customer

Asked: 2015-10-19

Answer

The products we sell, including anti-NKX6.3 antibody A30461, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-10-19

Question

Would A30461 anti-NKX6.3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

H. Li

Verified customer

Asked: 2013-04-19

Answer

It shows on the product datasheet, A30461 anti-NKX6.3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-04-19

Order DetailsPrice
A30461

100 μL

$335

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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