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Product Info Summary
| SKU: | M05891 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-NIT2 Mouse Monoclonal Antibody [Clone ID: OTI3D5]
SKU/Catalog Number
M05891
Size
100 µl
Description
Boster Bio Anti-NIT2 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5). Catalog# M05891. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-NIT2 Mouse Monoclonal Antibody [Clone ID: OTI3D5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05891)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3D5
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05891 is reactive to NIT2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
30.502kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05891 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either pCMV6-ENTRY NIT2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT2 mouse monoclonal (M05891)
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Anti-NIT2 mouse monoclonal antibody (M05891) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and NIT2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-NIT2 monoclonal antibody M05891
Protein Target Info & Infographic
Gene/Protein Information For NIT2 (Source: Uniprot.org, NCBI)
Gene Name
NIT2
Full Name
Omega-amidase NIT2
Weight
30.502kDa
Superfamily
carbon-nitrogen hydrolase superfamily
Alternative Names
Omega-amidase NIT2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NIT2, check out the NIT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NIT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NIT2 Mouse Monoclonal Antibody [Clone ID: OTI3D5] (M05891)
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