Product Info Summary
SKU: | A18099 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NHSL2 Antibody Picoband®
SKU/Catalog Number
A18099
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NHSL2 Antibody Picoband® catalog # A18099. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NHSL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18099)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NHSL2 recombinant protein (Position: Q596-D1042).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A18099 is reactive to NHSL2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
133 kDa
Calculated molecular weight
24145 MW
Background of NHSL2
Predicted to enable calcium ion binding activity. Predicted to be involved in cell differentiation. Predicted to be active in cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18099 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human SH-SY5Y whole cell, human RT4 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NHSL2 using anti-NHSL2 antibody (A18099).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHSL2 antigen affinity purified polyclonal antibody (Catalog # A18099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHSL2 at approximately 133 kDa. The expected band size for NHSL2 is at 133 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NHSL2 (Source: Uniprot.org, NCBI)
Gene Name
NHSL2
Full Name
NHS-like protein 2
Weight
24145 MW
Superfamily
NHS family
Alternative Names
Placenta-specific protein 9; PLAC9 NHSL2 NHS like 2 NHS-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NHSL2, check out the NHSL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NHSL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NHSL2 Antibody Picoband® (A18099)
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