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Product Info Summary
| SKU: | A08935-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NGEF Antibody Picoband®
SKU/Catalog Number
A08935-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NGEF Antibody Picoband® catalog # A08935-1. Tested in WB,FCM,ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NGEF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08935-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NGEF recombinant protein (Position: E82-Q627). Human NGEF shares 91.1% and 90.9% amino acid (aa) sequence identity with mouse and rat NGEF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08935-1 is reactive to NGEF in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
82,71 kDa
Background of NGEF
Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis. Predicted to be located in cytosol. Predicted to be active in glutamatergic synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08935-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human HepG2 whole cell, human Hela whole cell
FCM: HepG2 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NGEF using anti-NGEF antibody (A08935-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGEF antigen affinity purified polyclonal antibody (Catalog # A08935-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGEF at approximately 71 kDa. The expected band size for NGEF is at 82,71 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-NGEF antibody (A08935-1).
Overlay histogram showing HepG2 cells stained with A08935-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NGEF Antibody (A08935-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NGEF (Source: Uniprot.org, NCBI)
Gene Name
NGEF
Full Name
Ephexin-1
Weight
82,71 kDa
Alternative Names
EPHEXIN; ephexin-1; Eph-interacting exchange protein; neuronal guanine nucleotide exchange factorARHGEF27 NGEF ARHGEF27, EPHEXIN neuronal guanine nucleotide exchange factor ephexin-1|eph-interacting exchange protein|ephexin1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGEF, check out the NGEF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGEF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGEF Antibody Picoband® (A08935-1)
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