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Product Info Summary
| SKU: | A03765-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NFE2 Antibody Picoband®
View all Nuclear Factor Erythroid Derived 2 Antibodies
SKU/Catalog Number
A03765-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFE2 Antibody Picoband® catalog # A03765-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NFE2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03765-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFE2 recombinant protein (Position: E20-D373).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03765-1 is reactive to NFE2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
41.473kDa
Background of Nuclear Factor Erythroid Derived 2
Transcription factor NF-E2 45 kDa subunit is a protein that in humans is encoded by the NFE2 gene. Enables several functions, including WW domain binding activity; identical protein binding activity; and protein N-terminus binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development; negative regulation of bone mineralization; and negative regulation of syncytium formation by plasma membrane fusion. Part of protein-DNA complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03765-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NFE2 using anti-NFE2 antibody (A03765-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2 antigen affinity purified polyclonal antibody (Catalog # A03765-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2 at approximately 41 kDa. The expected band size for NFE2 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NFE2 (Source: Uniprot.org, NCBI)
Gene Name
NFE2
Full Name
Transcription factor NF-E2 45 kDa subunit
Weight
41.473kDa
Superfamily
bZIP family
Alternative Names
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4; SIRT4; SIR2L4 NFE2 NF-E2, p45 nuclear factor, erythroid 2 transcription factor NF-E2 45 kDa subunit|leucine zipper protein NF-E2|nuclear factor (erythroid-derived 2), 45kD|nuclear factor (erythroid-derived 2), 45kDa|nuclear factor, erythroid-derived 2 45 kDa subunit|p45 NF-E2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFE2, check out the NFE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFE2 Antibody Picoband® (A03765-1)
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