Anti-Neurotensin Receptor 1/NTSR1 Antibody Picoband™

Product Name

Anti-Neurotensin Receptor 1/NTSR1 Antibody Picoband™

View all NTS1/NTSR1 Antibodies

SKU/Catalog Number

A04610-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neurotensin Receptor 1/NTSR1 Antibody Picoband™ catalog # A04610-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Neurotensin Receptor 1/NTSR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04610-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Neurotensin Receptor 1/NTSR1 recombinant protein (Position: A15-D400).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04610-4 is reactive to NTSR1 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

43 kDa

Calculated molecular weight

Background of NTS1/NTSR1

Neurotensin receptor type 1 is a protein that in humans is encoded by the NTSR1 gene. Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04610-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Neurotensin Receptor 1/NTSR1 using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 antigen affinity purified polyclonal antibody (Catalog # A04610-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotensin Receptor 1/NTSR1 at approximately 43 kDa. The expected band size for Neurotensin Receptor 1/NTSR1 is at 43 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4).
Overlay histogram showing THP-1 cells stained with A04610-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 Antibody (A04610-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For NTSR1 (Source: Uniprot.org, NCBI)

Alternative Names

NTR1; NTRH; NTRR; NTS1; NTSR1 NTSR1 NTR neurotensin receptor 1 neurotensin receptor type 1|NT-R-1|NTR1|NTRH|high-affinity levocabastine-insensitive neurotensin receptor|neurotensin receptor 1 (high affinity)

For more info on NTSR1, check out the NTSR1 Infographic

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In this infographic, you will see the following information for NTSR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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